Anaerobic degradation of m-cresol by Desulfobacterium cetonicum is initiated by formation of 3-hydroxybenzylsuccinate

被引：56

作者：

Müller, JA ^{[1
]}

Galushko, AS ^{[1
]}

Kappler, A ^{[1
]}

Schink, B ^{[1
]}

机构：

[1] Univ Konstanz, Fak Biol, D-78457 Constance, Germany

来源：

ARCHIVES OF MICROBIOLOGY | 1999年 / 172卷 / 05期

关键词：

anaerobic degradation; aromatic compounds; m-cresol; toluene; 3-hydroxybenzylsuccinate; sulfate-reducing bacteria; Desulfobacterium cetonicum;

D O I：

10.1007/s002030050782

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The anaerobic bacterium Desulfobacterium cetonicum oxidized m-cresol completely with sulfate as electron acceptor. During growth, 3-hydroxybenzylsuccinate (identified by gas chromatography/mass spectroscopy and by comparison of high-performance liquid chromatography retention time and UV spectrum with a chemically synthesized reference compound) accumulated in the medium. This finding indicates that the methyl group of m-cresol is activated by addition to fumarate as in the case of anaerobic toluene metabolism. In cell-free extracts of D. cetonicum, the formation of 3-hydroxybenzylsuccinate from m-cresol and fumarate was detected at an activity of 0.5 nmol min(-1) (mg protein)(-1). This reaction depended strictly on anoxic assay conditions. Treatment with air resulted in a complete loss of activity; however, some activity could be recovered after restoring anoxic conditions. The activity was slightly membrane-associated. 3-Hydroxybenzylsuccinate was degraded via CoA thioesterification and further oxidation to 3-hydroxybenzoyl-CoA as subsequent steps in the degradation pathway.

引用

页码：287 / 294

页数：8

共 41 条

[1]

*AG TOX SUBST DIS, 1990, TOX PROF CRES

[2]

Barbour, 1984, MICROBIAL DEGRADATIO, P253

[3] ENZYMES OF THE REDUCTIVE CITRIC-ACID CYCLE IN THE AUTOTROPHIC EUBACTERIUM AQUIFEX-PYROPHILUS AND IN THE ARCHAEBACTERIUM THERMOPROTEUS-NEUTROPHILUS [J].