Improved methods for encoding and decoding dialkylamine-encoded combinatorial libraries

被引：32

作者：

Fitch, WL

Baer, TA

Chen, WW

Holden, F

Holmes, CP

Maclean, D

Shah, NH

Sullivan, E

Tang, M

Waybourn, P

Fischer, SM

Miller, CA

Synder, LR

机构：

[1] Affymax Res Inst, Santa Clara, CA 95051 USA

[2] Hewlett Packard Co, Palo Alto, CA 94304 USA

[3] LC Resources Inc, Orinda, CA 94563 USA

来源：

JOURNAL OF COMBINATORIAL CHEMISTRY | 1999年 / 1卷 / 03期

关键词：

D O I：

10.1021/cc990001r

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Optimized methods for utilizing and analyzing dialkylamine tags in encoded combinatorial chemistry are reported. A previously described LC/fluorescence method has been shortened from over 1 h to 6 min. A novel ion exchange LC/MS method for decoding has been developed which is both fast and rugged. The use of quantitative encoding schemes is described. These new techniques have allowed a simplified, automated sample preparation scheme, and over 300 encoded bead samples can be read in a day.

引用

页码：188 / 194

页数：7

共 12 条

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