Gene discovery in neuropharmacological and behavioral studies using Affymetrix microarray data

被引：13

作者：

Reimers, M ^{[1
]}

Heilig, M

Sommer, WH

机构：

[1] NIH, NCI, Mol Pharmacol Lab, Bethesda, MD 20892 USA

[2] NIAAA, Lab Clin & Translat Studies, NIH, Bethesda, MD USA

来源：

METHODS | 2005年 / 37卷 / 03期

关键词：

brain; expression microarray; regional hybridization bias; statistical analysis;

D O I：

10.1016/j.ymeth.2005.09.002

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe methods and software tools for doing data analysis based on Affymetrix microarray data, emphasizing often neglected issues. In our experience with neuroscience studies, experimental design and quality assessment are vital. We also describe in detail the pre-processing methods we have found useful for Affymetrix data. Finally, we summarize the statistical literature and describe some pitfalls in the post-processing analysis. (c) 2005 Elsevier Inc. All rights reserved.

引用

页码：219 / 228

页数：10

共 26 条

[1] A cluster of differentially expressed signal transduction genes identified by microarray analysis in a rat genetic model of alcoholism [J].