An original transesterification route for fatty acid ester production from vegetable oils in a solvent-free system

被引:16
作者
GomaDoncescu, N
Legoy, MD
机构
[1] Lab. Genie Proteique et Cell., Pôle Sciences et Technologie
[2] Lab. Genie Proteique et Cell., Pôle Sciences et Technologie, F-17 042 La Rochelle Cedex 01, Avenue Marillac
关键词
lipozyme; solvent-free system; transesterification; vegetable oils; wax esters;
D O I
10.1007/s11746-997-0037-9
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
Wax ester production from a long-chain alcohol and methyl ester has been investigated with an immobilized thermostable lipase (lipozyme IM from Novo Nordisk). The transesterification reaction rate was monitored in solvent-free medium that was exclusively composed of the reactants and the enzyme. The transesterification is performed by simply mixing the two substrates in various stoichiometric amounts at a temperature range from 55 to 65 degrees C under constant stirring in the presence of low concentrations of enzyme preparation (0.12 to 2%, w/w), Long-chain reactants produce waxes of high molecular mass that induce low solubility and high viscosity. On average, high transesterification yields are obtained (around 95%). Thermodynamic parameters involving substrate concentration and temperature have also been investigated. The balance between optimal working temperature and the molar ratio of substrates in such a complex medium appears to be 60 degrees C, with a molar ratio methyl oleate/stearyl alcohol of 1:0.5. Substrate in hibition due to stearyl alcohol has been observed. A study of kinetic parameters has confirmed these results.
引用
收藏
页码:1137 / 1143
页数:7
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