Circulating miRNAs expression profiling in drug-resistant epilepsy: Up-regulation of miR-301a-3p in a case of sudden unexpected death

被引:13
作者
De Matteis, Maria [1 ]
Cecchetto, Giovanni [1 ]
Munari, Giada [2 ]
Balsamo, Laura [2 ]
Gardiman, Marina Paola [3 ]
Giordano, Renzo [4 ]
Viel, Guido [1 ]
Fassan, Matteo [2 ]
机构
[1] Univ Padua, Dept Legal & Occupat Med, Toxicol & Publ Hlth, Via Falloppio 50, I-35121 Padua, Italy
[2] Univ Padua, Surg Pathol Unit, Dept Med DIMED, Padua, Italy
[3] Padua Univ Hosp, Surg Pathol Unit, Via Giustiniani 2, I-35121 Padua, Italy
[4] ULSS 13 Dolo Hosp, Surg Pathol Unit, Dolo, Venice, Italy
关键词
Forensic preventative pathology; MiRNA; Biomarkers; Epilepsy; Sudden death; PREVENTION; MECHANISMS; MICRORNAS; MOLECULES;
D O I
10.1016/j.legalmed.2017.12.003
中图分类号
DF [法律]; D9 [法律]; R [医药、卫生];
学科分类号
030101 [法学理论]; 030111 [法律史学]; 100218 [急诊医学];
摘要
Sudden and unexpected death in epilepsy (SUDEP) represents one of the most challenging fields for clinical, forensic and preventative pathology. Several authors have emphasized the search of innovative biomarkers related to drug-resistance for an appropriate risk stratification in these patients. However, no reliable biomarker has been implemented into clinical practice, so far. Herein, we present a case of SUDEP due to drug-resistant mesial temporal lobe epilepsy (MTLE) in which we performed miRNA expression profiling (miR-301a-3p, miR-194-5p, miR-30b-5p, mIR-342-5p, and miR-4446-3p) from both the plasma and the temporal lobe in comparison to ten autopsies for traumatic or asphyxia deaths. A significant up-regulation of miR-301a-3p in both the plasma (2.3 increase vs. controls) and the hippocampus (3.2-fold increase vs. controls) was evidenced, whereas the other tested miRNAs showed no significant expression differences between case and controls. Even preliminary, our results support miRNAs as an innovative class of biomarkers compatible with an adequate analysis of biospecimens obtained from forensic autopsies.
引用
收藏
页码:7 / 9
页数:3
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