Decoding enhancers using massively parallel reporter assays

被引：176

作者：

Inoue, Fumitaka

Ahituv, Nadav ^{[1
]}

机构：

[1] Univ Calif San Francisco, Dept Bioengn & Therapeut Sci, San Francisco, CA 94158 USA

来源：

GENOMICS | 2015年 / 106卷 / 03期

关键词：

Enhancer; Massively parallel reporter assays; Transcriptional regulation; TISSUE-SPECIFIC ENHANCERS; REGULATORY ELEMENTS; OPEN CHROMATIN; ACTIVITY MAPS; HUMAN GENOME; IN-VIVO; SYSTEMATIC DISSECTION; PROMOTERS; SEQUENCES; SEQ;

D O I：

10.1016/j.ygeno.2015.06.005

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Enhancers control the timing, location and expression levels of their target genes. Nucleotide variation in enhancers has been shown to lead to numerous phenotypes, including human disease. While putative enhancer sequences and nucleotide variation within them can now be detected in a rapid manner using various genomic technologies, the understanding of the functional consequences of these variants still remains largely unknown. Massively parallel reporter assays (MPRAs) can overcome this hurdle by providing the ability to test thousands of sequences and nucleotide variants within them for enhancer activity en masse. Here, we describe this technology and specifically focus on how it is being used to obtain an increased understanding of enhancer regulatory code and grammar. (C) 2015 Elsevier Inc. All rights reserved.

引用

页码：159 / 164

页数：6

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