Development and evaluation of a new personal sampler for culturable airborne microorganisms

被引:58
作者
Agranovski, IE [1 ]
Agranovski, V
Reponen, T
Willeke, K
Grinshpun, SA
机构
[1] Griffith Univ, Fac Environm Sci, Brisbane, Qld 4111, Australia
[2] Queensland Univ Technol, Ctr Med Hlth & Environm Phys, Brisbane, Qld, Australia
[3] Univ Cincinnati, Dept Environm Hlth, Ctr Hlth Related Aerosol Studies, Cincinnati, OH 45267 USA
关键词
bioaerosol; personal sampling; viable microorganisms; liquid collection; collection efficiency;
D O I
10.1016/S1352-2310(01)00488-5
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
The objective of this study was to develop a new personal sampler for viable airborne microorganisms and to evaluate its performance under controlled laboratory conditions and in a field. In the sampler, air is bubbled through a porous medium submerged in a liquid layer, as has earlier been demonstrated to be highly efficient for air purification. The prototype had the physical collection efficiency > 95% for particles > 0.32 mum in aerodynamic diameter during 8 h of continuous operation. The pressure drop across the sampler was below 1700 Pa. much lower than that of most conventional bioaerosol samplers. The collection liquid losses due to evaporation and aerosolization did not exceed 18% in 8 h and the culturability of sampled microorganisms remained high: the recovery rate of stress-sensitive gram-negative P. fluorescens bacteria was 61 +/- 20%; for stress-resistant B. subtilis bacteria and A. versicolor fungal spores it was 95 +/- 9% and 97 +/- 6%, respectively. Six identical personal samplers were tested simultaneously on a simplified human manikin in an office environment. The culturable microbial concentration data obtained during 2, 4 and 8-h sampling were not affected by the sampling time. Inter-sample variation did not exceed 30%. The laboratory and field evaluations have demonstrated that the new sampler is capable of long-term personal sampling of airborne culturable microorganisms. The estimation of the detection limits has indicated that the sampler is capable of monitoring microbial exposure in the environments with the bacterial concentrations above 15 CFU/m(3) and fungal concentrations above 5 CFU/m(3) when using a sampling time of 8 h. (C) 2002 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:889 / 898
页数:10
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