Rapid Staphylococcus aureus agr type determination by a novel multiplex real-time quantitative PCR assay

被引:32
作者
Francois, P [1 ]
Koessler, T
Huyghe, A
Harbarth, S
Bento, M
Lew, D
Etienne, J
Pittet, D
Schrenzel, J
机构
[1] Univ Hosp Geneva, Infect Dis Serv, Genom Res Lab, CH-1211 Geneva 14, Switzerland
[2] Univ Hosp Geneva, Infect Dis Serv, Infect Control Program, CH-1211 Geneva, Switzerland
[3] Univ Hosp Geneva, Infect Dis Serv, Clin Microbiol Lab, CH-1211 Geneva 14, Switzerland
[4] Ctr Natl Reference Staphylocoques, INSERM, E0230, Lyon, France
关键词
D O I
10.1128/JCM.44.5.1892-1895.2006
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The accessory gene regulator (agr) is a crucial regulatory component of Staphylococcus aureus involved in the control of bacterial virulence factor expression. We developed a real-time multiplex quantitative PCR assay for the rapid determination of S. aureus agr type. This assay represents a rapid and affordable alternative to sequence-based strategies for assessing relevant epidemiological information.
引用
收藏
页码:1892 / 1895
页数:4
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