Fungal pectic enzyme fractionation by dye affinity chromatography

被引：8

作者：

Camperi, SA ^{[1
]}

Grasselli, M ^{[1
]}

Cascone, O ^{[1
]}

机构：

[1] UNIV BUENOS AIRES, FAC FARM & BIOQUIM, CATEDRA MICROBIOL IND & BIOTECNOL, RA-1113 BUENOS AIRES, DF, ARGENTINA

来源：

BIOTECHNOLOGY TECHNIQUES | 1996年 / 10卷 / 03期

关键词：

D O I：

10.1007/BF00158948

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

A dye affinity fractionation of a commercial pectic enzyme preparation is described. From nine immobilized triazinic dyes assayed, only Blue R-HE and Yellow 4R-HE were able to retain pectin lyase thus allowing the obtention of a fraction not producing methanol during fruit juice clarification. While pectinesterase did not bind to the chromatographic matrix, the retained pectin lyase could be quantitatively eluted with a salt gradient.

引用

页码：211 / 214

页数：4

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