Propofol induces ERK-dependant expression of c-Fos and Egr-1 in neuronal cells

被引：19

作者：

Kidambi, Srivatsan ^{[1
,2
,3
]}

Yarmush, Joel ^{[1
]}

Fong, Wayne ^{[1
]}

Kamath, Sangeetha ^{[1
]}

SchianodiCola, Joseph ^{[1
]}

Nahmias, Yaakov ^{[2
,3
]}

机构：

[1] New York Methodist Hosp, Dept Anesthesiol, Brooklyn, NY USA

[2] Shriners Hosp Children, Massachusetts Gen Hosp, Ctr Engn Med & Surg Serv, Boston, MA USA

[3] Harvard Univ, Sch Med, Boston, MA USA

来源：

NEUROREPORT | 2009年 / 20卷 / 07期

关键词：

gamma-aminobutyric acid; extracellular signal-regulated kinase; immediate early genes; propofol; IMMEDIATE-EARLY GENES; RAT HIPPOCAMPAL SLICES; LONG-TERM POTENTIATION; PROTEIN-KINASES; ACTIVATION; MEMORY; PHOSPHORYLATION; ANESTHETICS; ISOFLURANE;

D O I：

10.1097/WNR.0b013e328329a449

中图分类号：

Q189 [神经科学];

学科分类号：

071006 [神经生物学];

摘要：

This study explored the effects of propofol on c-Fos and Egr-1 in neuroblastoma (N2A) cells. We demonstrate that propofol induced the expression of c-Fos and Egr-1 within 30 and 60 min of exposure time. At 16.8 mu M concentration, propofol induced a 6 and 2.5-fold expression of c-Fos and Egr-1, respectively. However, at concentrations above 100 mu M, propofol failed to induce expression of c-Fos or Egr-1. Propofol-induced c-Fos and Egr-1 transcription was unaffected by bicuculline, a gamma-aminobutyric acid-A receptor antagonist, but was abolished by PD98059, a mitogen-activated protein kinase/extracellular signal-regulated kinase inhibitor. Our study shows that clinically relevant concentrations of propofol induce c-Fos and Egr-1 expression through an extracellular signal-regulated kinase mediated and gamma-aminobutyric acid-A independent pathway. NeuroReport 20:657-662 (C) 2009 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins.

引用

页码：657 / 662

页数：6

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