Perspectives on Mucus Properties and Formation-Lessons from the Biochemical World

被引：47

作者：

Ambort, Daniel ^{[1
]}

Johansson, Malin E. V. ^{[1
]}

Gustafsson, Jenny K. ^{[1
]}

Ermund, Anna ^{[1
]}

Hansson, Gunnar C. ^{[1
]}

机构：

[1] Univ Gothenburg, Dept Med Biochem, S-40530 Gothenburg, Sweden

来源：

COLD SPRING HARBOR PERSPECTIVES IN MEDICINE | 2012年 / 2卷 / 11期

基金：

瑞典研究理事会;

关键词：

VON-WILLEBRAND-FACTOR; RAT SMALL-INTESTINE; HUMAN MUC5AC MUCIN; LS 174T CELLS; BICARBONATE SECRETION; O-GLYCOSYLATION; CYSTIC-FIBROSIS; INSOLUBLE MUCIN; C-TERMINUS; IDENTIFICATION;

D O I：

10.1101/cshperspect.a014159

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

100103 [病原生物学]; 100218 [急诊医学];

摘要：

Our model of the MUC2 mucin shows a well-organized netlike gel that is cross-linked by six different covalent and noncovalent bonds. When the MUC2 mucin is packed in the mucin granule it is organized by an amino-terminal concatenated ring platform formed at high calcium and low pH. This packing allows an ordered release and a normal mucin expansion when calcium is removed and pH increased by bicarbonate. This process is defective in the absence of cystic fibrosis transmembrane conductance regulator (CFTR)-dependent bicarbonate transport. The expanded secreted mucin is suggested to be self-organizing by properties inherited in the MUC2 mucin and by proteolytic processes.

引用

页数：9

共 37 条

[1]

Calcium and pH-dependent packing and release of the gel-forming MUC2 mucin [J].

Ambort, Daniel ;

Johansson, Malin E. V. ;

Gustafsson, Jenny K. ;

Nilsson, Harriet E. ;

Ermund, Anna ;

Johansson, Bengt R. ;

Koeck, Philip J. B. ;

Hebert, Hans ;

Hansson, Gunnar C. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2012, 109 (15) :5645-5650

[2]

Function of the CysD domain of the gel-forming MUC2 mucin [J].

Ambort, Daniel ;

van der Post, Sjoerd ;

Johansson, Malin E. V. ;

MacKenzie, Jenny ;

Thomsson, Elisabeth ;

Krengel, Ute ;

Hansson, Gunnar C. .

BIOCHEMICAL JOURNAL, 2011, 436 :61-70

[3]

Large scale identification of proteins, mucins, and their O-glycosylation in the endocervical mucus during the menstrual cycle [J].

Andersch-Bjorkman, Ylva ;

Thomsson, Kristina A. ;

Larssont, Jessica M. Holmen ;

Ekerhovd, Erling ;

Hansson, Gunnar C. .

MOLECULAR & CELLULAR PROTEOMICS, 2007, 6 (04) :708-716

[4]

Human MUC5AC mucin dimerizes in the rough endoplasmic reticulum, similarly to the MUC2 mucin [J].

Asker, N ;

Axelsson, MAB ;

Olofsson, SO ;

Hansson, GC .

BIOCHEMICAL JOURNAL, 1998, 335 :381-387

[5]

THE HUMAN MUC2 MUCIN APOPROTEIN APPEARS TO DIMERIZE BEFORE O-GLYCOSYLATION AND SHARES EPITOPES WITH THE INSOLUBLE MUCIN OF RAT SMALL-INTESTINE [J].

ASKER, N ;

BAECKSTROM, D ;

AXELSSON, MAB ;

CARLSTEDT, I ;

HANSSON, GC .

BIOCHEMICAL JOURNAL, 1995, 308 :873-880

[6]

The adherent gastrointestinal mucus gel layer: thickness and physical state in vivo [J].

Atuma, C ;

Strugala, V ;

Allen, A ;

Holm, L .

AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY, 2001, 280 (05) :G922-G929

[7]

O-glycosylated MUC2 monomer and dimer from LS 174T cells are water-soluble, whereas larger MUC2 species formed early during biosynthesis are insoluble and contain nonreducible intermolecular bonds [J].

Axelsson, MAB ;

Asker, N ;

Hansson, GC .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (30) :18864-18870

[8]

CARLSTEDT I, 1993, J BIOL CHEM, V268, P18771

[9]

Evolutionary considerations in relating oligosaccharide diversity to biological function [J].

Gagneux, P ;

Varki, A .

GLYCOBIOLOGY, 1999, 9 (08) :747-755

[10]

Normal mouse intestinal mucus release requires cystic fibrosis transmembrane regulator-dependent bicarbonate secretion [J].

Garcia, Mary Abigail S. ;

Yang, Ning ;

Quinton, Paul M. .

JOURNAL OF CLINICAL INVESTIGATION, 2009, 119 (09) :2613-2622

← 1 2 3 4 →