Pectin esterase gene family in strawberry fruit:: study of FaPE1, a ripening-specific isoform

被引:112
作者
Castillejo, C
de la Fuente, JI
Iannetta, P
Botella, MA
Valpuesta, V
机构
[1] Univ Malaga, Fac Ciencias, Dept Bioquim & Biol Mol, E-29071 Malaga, Spain
[2] Scottish Crop Res Inst, Dundee DD2 4DN, Scotland
关键词
auxin; cell wall; ethylene; fruit ripening; pectin esterase; strawberry;
D O I
10.1093/jxb/erh102
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Pectin esterases (PE, EC 3.1.1.11) catalyse the demethylation of pectin. As a result of its activity, structural interactions among cell wall components during cell wall turnover and loosening are affected. In plants, PEs are typically encoded by a gene family. This family has been studied in strawberry (Fragariaxananassa Duch.) in order to investigate the role of distinct PE genes during fruit ripening and senescence. By a combination of a PCR-based library screening and RT-PCR four different strawberry PE cDNAs, termed FaPE1 to FaPE4, have been isolated. Differential expression of each FaPE gene in various organs and during fruit development was revealed by northern blot. FaPE1 is specifically expressed in fruit, showing an increasing expression during the ripening process up to a maximum in the turning stage. Concerning hormone regulation, auxin treatment increased FaPE1 mRNA levels in green fruit, whereas exogenous ethylene decreased FaPE1 mRNA levels in ripe and senescing fruits. It is proposed that this repression of FaPE1 expression could be involved in textural changes occurring during fruit senescence.
引用
收藏
页码:909 / 918
页数:10
相关论文
共 54 条
[1]  
AGINS F, 2003, NAT BIOTECHNOL, V21, P177
[2]   Novel insight into vascular, stress, and auxin-dependent and -independent gene expression programs in strawberry, a non-climacteric fruit [J].
Aharoni, A ;
Keizer, LCP ;
Van den Broeck, HC ;
Blanco-Portales, R ;
Muñoz-Blanco, J ;
Bois, G ;
Smit, P ;
De Vos, RCH ;
O'Connell, AP .
PLANT PHYSIOLOGY, 2002, 129 (03) :1019-1031
[3]   Gene expression analysis of strawberry achene and receptacle maturation using DNA microarrays [J].
Aharoni, A ;
O'Connell, AP .
JOURNAL OF EXPERIMENTAL BOTANY, 2002, 53 (377) :2073-2087
[4]   A GENE SHOWING SEQUENCE SIMILARITY TO PECTIN ESTERASE IS SPECIFICALLY EXPRESSED IN DEVELOPING POLLEN OF BRASSICA-NAPUS - SEQUENCES IN ITS 5' FLANKING REGION ARE CONSERVED IN OTHER POLLEN-SPECIFIC PROMOTERS [J].
ALBANI, D ;
ALTOSAAR, I ;
ARNISON, PG ;
FABIJANSKI, SF .
PLANT MOLECULAR BIOLOGY, 1991, 16 (04) :501-513
[5]  
ALMEIDA DPF, 1999, PHYSIOL PLANTARUM, V104, P105
[6]   A PUTATIVE VACUOLAR PROCESSING PROTEASE IS REGULATED BY ETHYLENE AND ALSO DURING FRUIT RIPENING IN CITRUS-FRUIT [J].
ALONSO, JM ;
GRANELL, A .
PLANT PHYSIOLOGY, 1995, 109 (02) :541-547
[7]  
ARCHBOLD DD, 1984, J AM SOC HORTIC SCI, V109, P330
[8]   CELL-WALL DEGRADING ENZYMES AND SOFTENING OF SENESCENT STRAWBERRY FRUIT [J].
BARNES, MF ;
PATCHETT, BJ .
JOURNAL OF FOOD SCIENCE, 1976, 41 (06) :1392-1395
[9]   Pectinmethylesterase isoforms from Vigna radiata hypocotyl cell walls: Kinetic properties and molecular cloning of a cDNA encoding the most alkaline isoform [J].
Bordenave, M ;
Breton, C ;
Goldberg, R ;
Huet, JC ;
Perez, S ;
Pernollet, JC .
PLANT MOLECULAR BIOLOGY, 1996, 31 (05) :1039-1049
[10]  
BORDENAVE M, 1996, MOD METHOD PLANT, V17, P165