Molecular differentiation between NS1 gene of a field strain Bluetongue virus serotype 2 (BTV-2) and NS1 gene of an attenuated BTV-2 vaccine

被引：23

作者：

Agüero, M ^{[1
]}

Arias, M ^{[1
]}

Romero, LJ ^{[1
]}

Zamora, MJ ^{[1
]}

Sánchez-Vizcaíno, JM ^{[1
]}

机构：

[1] CISA, INIA, Voldeolmos Madrid 28130, Spain

来源：

VETERINARY MICROBIOLOGY | 2002年 / 86卷 / 04期

关键词：

Bluetongue virus; molecular diagnosis; RT-PCR; vaccine;

D O I：

10.1016/S0378-1135(02)00011-1

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

At the end of September 2000, clinical symptoms of Bluetongue appeared in sheep flocks of the Balearic Islands (Spain). The presence of the BTV serotype 2 in tissue and blood samples of affected animals was confirmed by laboratory techniques. A systematic vaccination were carried out in affected areas using a live monovalent serotype 2 vaccine available from Onderstepoort laboratory (South Africa). In order to perform epidemiological studies, a new method to differentiate between the NS1 genes of BTV-2 affecting the Balearic islands and that of the Onderstepoort commercial live virus vaccine (monovalent, serotype 2) has been developed. This procedure is based on the use of an RT-PCR, followed by restriction endonuclease analysis. Epidemiological data of a study carried out in the period January-October 2001 using this procedure are included. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：337 / 341

页数：5

共 10 条

[1] IDENTIFYING BLUETONGUE VIRUS-RIBONUCLEIC-ACID SEQUENCES BY THE POLYMERASE CHAIN-REACTION [J].