A link between ferritin gene expression and ribonucleotide reductase R2 protein, as demonstrated by retroviral vector mediated stable expression of R2 cDNA

被引：15

作者：

Fan, HZ

Villegas, C

Wright, JA

机构：

[1] MANITOBA INST CELL BIOL,WINNIPEG,MB R3E 0V9,CANADA

[2] UNIV MANITOBA,WINNIPEG,MB R3E 0V9,CANADA

来源：

FEBS LETTERS | 1996年 / 382卷 / 1-2期

关键词：

ferritin; DNA synthesis; ribonucleotide reductase; gene expression;

D O I：

10.1016/0014-5793(96)00143-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have constructed a retroviral expression vector for the mammalian ribonucleotide reductase R2 component, Stable infectants, which express a myc epitope tagged R2 protein from the vector cDNA were obtained and described for the first time, Cells containing the recombinant protein exhibited increased ribonucleotide reductase activity, and were resistant to the antitumour agent hydroxyurea, which targets the R2 component of ribonucleotide reductase. Furthermore, a direct link between ferritin gene expression and R2 protein was observed, since cells containing vector expressed recombinant R2 protein exhibited H-chain and L-chain ferritin gene expression.

引用

页码：145 / 148

页数：4

共 15 条

[1] THE GENE FOR A NOVEL PROTEIN, A MEMBER OF THE PROTEIN DISULFIDE ISOMERASE/FORM-I PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE-C FAMILY, IS AMPLIFIED IN HYDROXYUREA-RESISTANT CELLS [J].