Kaempferol induces apoptosis in human HCT116 colon cancer cells via the Ataxia-Telangiectasia Mutated-p53 pathway with the involvement of p53 Upregulated Modulator of Apoptosis

被引：83

作者：

Li, Wei ^{[1
]}

Du, Bingna ^{[1
]}

Wang, Tianyi ^{[1
]}

Wang, Siling ^{[2
]}

Zhang, Jinghai ^{[1
]}

机构：

[1] Shenyang Pharmaceut Univ, Sch Life Sci & Biopharmaceut, Biochem Lab, Shenyang 110016, Peoples R China

[2] Shenyang Pharmaceut Univ, Sch Pharm, Shenyang 110016, Peoples R China

来源：

CHEMICO-BIOLOGICAL INTERACTIONS | 2009年 / 177卷 / 02期

关键词：

Colorectal cancer p53; ATM (Ataxia-Telangiectasia Mutated); Kaempferol; PUMA (p53 Upregulated Modulator of Apoptosis); Apoptosis; NEGATIVE PROGNOSTIC-FACTOR; STRAND BREAK REPAIR; COLORECTAL-CANCER; DIETARY FLAVONOIDS; DNA-DAMAGE; LOW BAX; INHIBITION; GROWTH; PROLIFERATION; RISK;

D O I：

10.1016/j.cbi.2008.10.048

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Dietary flavonols have been found to possess preventive and therapeutic potential against several kinds of cancers. This study is conducted to investigate the anti-proliferation effects of kaempferol, a major component of food flavonols. against colon cancer cells. In the human HCT116 colon cancer cell line, kaempferol induced p53-dependent growth inhibition and apoptosis. Furthermore, kaempferol was found to induce cytochrome c release from mitochondria and activate caspase-3 cleavage. The Bcl-2 family proteins including PUMA were involved in this process. Kaempferol also induced ATM and H2AX phosphorylation in HCT116 cells, inhibition of ATM by a chemical inhibitor resulted in abrogation of the downstream apoptotic cascades. These findings Suggest kaempferol could be a potent candidate for colorectal cancer management. (C) 2008 Elsevier Ireland Ltd. All rights reserved.

引用

页码：121 / 127

页数：7

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