Trichinella spiralis: Synthesis of type IV and type VI collagen during nurse cell formation

被引:36
作者
Polvere, RI
Kabbash, CA
Capo, VA
Kadan, I
Despommier, DD
机构
[1] COLUMBIA UNIV,DIV ENVIRONM HLTH SCI,NEW YORK,NY 10032
[2] COLUMBIA UNIV,DEPT MICROBIOL,NEW YORK,NY 10032
[3] COLUMBIA UNIV,DEPT PHYSIOL,NEW YORK,NY 10032
关键词
Trichinella spiralis; collagen; in situ hybridization; host-parasite relationship; Northern; Nurse cell; BCIP; 5-bromo-4-chloro-3-indolyl phosphate;
D O I
10.1006/expr.1997.4180
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
The portion of skeletal muscle fiber (Nurse cell) harboring Trichinella spiralis is surrounded by an acellular capsule susceptible to digestion with collagenase. Antibodies recognized type IV and type VI collagen in the capsule, while the periodic acid Schiff reagent stained the capsule differentially, revealing at least two distinct layers. RNA analysis showed that mRNA specific for type IV and type VI collagen was present in muscle tissue on Days 9 and 15, but not on Day 3, following intracellular infection. In situ hybridization showed that most of the mRNA for both types was within the Nurse cell, and all enlarged Nurse cell nuclei were transcriptionally active for those messages. Synthesis of type IV collagen mRNA was absent by Day 24. In contrast, type VI collagen mRNA was still present at 24 days and 8 months. These results support the hypothesis that T. spiralis, either directly or indirectly, influences the synthesis of these two collagen types throughout its own developmental cycle in the Nurse cell. (C) 1997 Academic Press.
引用
收藏
页码:191 / 199
页数:9
相关论文
共 36 条
[1]   BLOOD-VESSELS IN TRICHINELLA-SPIRALIS INFECTIONS - A STUDY USING VASCULAR CASTS [J].
BARUCH, AM ;
DESPOMMIER, DD .
JOURNAL OF PARASITOLOGY, 1991, 77 (01) :99-103
[2]   SOME OBSERVATIONS ON FINE STRUCTURE OF MATURE LARVA OF NEMATODE TRICHINELLA SPIRALIS [J].
BECKETT, EB ;
BOOTHROYD, B .
ANNALS OF TROPICAL MEDICINE AND PARASITOLOGY, 1961, 55 (01) :116-&
[3]  
BOCZON K, 1989, Wiadomosci Parazytologiczne, V35, P435
[4]  
CHOMCZYNSKI P, 1993, BIOTECHNIQUES, V15, P532
[5]   FERRITIN-CONJUGATED ANTIBODY STUDIES ON LARVAE OF TRICHINELLA SPIRALIS [J].
DESPOMMI.DD ;
KAJIMA, M ;
WORSTMAN.BS .
JOURNAL OF PARASITOLOGY, 1967, 53 (03) :618-+
[6]  
DESPOMMIER D, 1975, AM J PATHOL, V78, P477
[7]   TRICHINELLA-SPIRALIS - GROWTH OF INTRACELLULAR (MUSCLE) LARVA [J].
DESPOMMIER, D ;
ARON, L ;
TURGEON, L .
EXPERIMENTAL PARASITOLOGY, 1975, 37 (01) :108-116
[8]   CHANGES IN NURSE CELL-NUCLEI DURING SYNCHRONOUS INFECTION WITH TRICHINELLA-SPIRALIS [J].
DESPOMMIER, D ;
SYMMANS, WF ;
DELL, R .
JOURNAL OF PARASITOLOGY, 1991, 77 (02) :290-295
[9]   TRICHINELLA-SPIRALIS AND THE CONCEPT OF NICHE [J].
DESPOMMIER, DD .
JOURNAL OF PARASITOLOGY, 1993, 79 (04) :472-482
[10]   TRICHINELLA-SPIRALIS - SECRETED ANTIGEN OF THE INFECTIVE L1 LARVA LOCALIZES TO THE CYTOPLASM AND NUCLEOPLASM OF INFECTED HOST-CELLS [J].
DESPOMMIER, DD ;
GOLD, AM ;
BUCK, SW ;
CAPO, V ;
SILBERSTEIN, D .
EXPERIMENTAL PARASITOLOGY, 1990, 71 (01) :27-38