Next-generation sequencing technologies in diagnostic virology

被引:85
作者
Barzon, Luisa [1 ]
Lavezzo, Enrico [1 ]
Costanzi, Giulia [1 ]
Franchin, Elisa [1 ]
Toppo, Stefano [1 ]
Palu, Giorgio [1 ]
机构
[1] Univ Padua, Dept Mol Med, I-35121 Padua, Italy
关键词
Genome sequencing; Bioinformatics; Deep/high throughput sequencing; Virus discovery; Viral metagenomices/viromics; Viral epidemiology; FEBRILE ILLNESS; VIRUS; REVEALS; IDENTIFICATION; DYNAMICS;
D O I
10.1016/j.jcv.2013.03.003
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The data deluge produced by next-generation sequencing (NGS) technologies is an appealing feature for clinical virologists that are involved in the diagnosis of emerging viral infections, molecular epidemiology of viral pathogens, drug-resistance testing, and also like to do some basic and clinical research. Indeed, NGS platforms are being implemented in many clinical and research laboratories, as the costs of these platforms are progressively decreasing. We provide here some suggestions for virologists who are planning to implement a NGS platform in their clinical laboratory and an overview on the potential applications of these technologies in diagnostic virology. (c) 2013 Elsevier B.V. All rights reserved.
引用
收藏
页码:346 / 350
页数:5
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