Pertussis toxin decreases absence seizures and GABAB receptor binding in thalamus of a genetically prone rat (GAERS)

被引:9
作者
Bowery, NG [1 ]
Parry, K
Boehrer, A
Mathivet, P
Marescaux, C
Bernasconi, R
机构
[1] Univ Birmingham, Sch Med, Dept Pharmacol, Birmingham B15 2TT, W Midlands, England
[2] Hospices Civils Strasbourg, Neurol Clin, F-67091 Strasbourg, France
关键词
GABA(B); pertussis toxin; absence seizures; GAERS;
D O I
10.1016/S0028-3908(99)00118-5
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Postsynaptic GABA(B) receptor-mediated events have previously been shown to be reduced by prior treatment with pertussis toxin in rat brain. In the present study genetic absence epilepsy rats from Strasbourg (GAERS) were given single bilateral injections of pertussis toxin (PTx 0.4 mu g), denatured-PTx or vehicle saline into the relay nuclei of the thalamus under anaesthesia. After recovery the spike and wave discharge duration (SWD) was monitored for up to 6 days following which the brains were removed and GABA(B) or GABA(A) receptor autoradiography performed on TO mu m transverse sections. By 6 days the SWD of the rats treated with PTx was suppressed by 96% compared with vehicle-injected rats with a significant (62%) reduction even after 1 day. Denatured toxin had no effect at any time. After 6 days GABA(B), but not GABA(A), receptor binding was significantly reduced by 70-80% in the ventrolateral and ventral posteriolateral thalamic nuclei. No changes in other brain regions were detected and denatured toxin failed to alter GABA(A) or GABA(B) receptor binding in any brain region. These data implicate G-protein mechanisms in the generation of SWD in GAERS and support the role of GABA(B) receptors in their induction within the thalamus. (C) 1999 Elsevier;Science Ltd, All rights reserved.
引用
收藏
页码:1691 / 1697
页数:7
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