Tetracycline suppresses ATPγS-induced CXCL8 and CXCL1 production by the human dermal microvascular endothelial cell-1 (HMEC-1) cell line and primary human dermal microvascular endothelial cells

被引:24
作者
Bender, Anna [1 ]
Zapolanski, Tamar [1 ]
Watkins, Shannon [1 ]
Khosraviani, Ava [1 ]
Seiffert, Kristina [1 ]
Ding, Wanhong [1 ]
Wagner, John A. [2 ]
Granstein, Richard D. [1 ]
机构
[1] Cornell Univ, Weill Med Coll, Dept Dermatol, New York, NY 10021 USA
[2] Cornell Univ, Weill Med Coll, Dept Neurol & Neurosci, New York, NY 10021 USA
关键词
adenosine triphosphate; chemokines; cytokines; endothelial cells; tetracycline;
D O I
10.1111/j.1600-0625.2008.00716.x
中图分类号
R75 [皮肤病学与性病学];
学科分类号
100206 ;
摘要
Tetracyclines (TCN) have powerful anti-inflammatory properties in addition to their anti-microbial effects. These anti-inflammatory effects are thought to play a role in inhibiting cutaneous inflammation in patients with rosacea and acne; however, the mechanism(s) of this action remains poorly understood. We have previously shown that adenosine-5'-triphosphate (ATP)gamma S, a hydrolysis-resistant ATP analogue, augments secretion of pro-inflammatory messengers by a human dermal microvascular endothelial cell line (HMEC-1). ATP released by the sympathetic nerves during stress may stimulate release of pro-inflammatory chemokines by dermal vessel endothelial cells, resulting in recruitment of inflammatory cells and exacerbation of inflammatory skin disease. Here we demonstrate that TCN inhibits ATP gamma S-induced release of pro-inflammatory mediators by HMEC-1 cells and primary human dermal microvascular endothelial cells. TCN dose-dependently inhibited ATP gamma S-induced augmentation of CXCL8 (interleukin-8) and CXCL1 (growth-regulated oncogene-alpha) production by HMEC-1 cells and primary human dermal endothelial cells in vitro. TCN and ATP gamma S did not affect HMEC-1 cell viability as determined by trypan-blue exclusion and cell counts. Inhibition of production of inflammatory mediators by endothelial cells may be one mechanism by which TCN improves inflammatory skin diseases. The ability to inhibit release of inflammatory mediators induced in HMEC-1 cells by purinergic agonists may be a useful way to screen for potential therapeutic agents for cutaneous inflammation.
引用
收藏
页码:752 / 760
页数:9
相关论文
共 49 条
[1]   HMEC-1 - ESTABLISHMENT OF AN IMMORTALIZED HUMAN MICROVASCULAR ENDOTHELIAL-CELL LINE [J].
ADES, EW ;
CANDAL, FJ ;
SWERLICK, RA ;
GEORGE, VG ;
SUMMERS, S ;
BOSSE, DC ;
LAWLEY, TJ .
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1992, 99 (06) :683-690
[2]  
AKAMATSU H, 1992, ACTA DERM-VENEREOL, V72, P178
[3]   Post-transcriptional regulation of inducible nitric oxide synthase mRNA in murine macrophages by doxycycline and chemically modified tetracyclines [J].
Amin, AR ;
Patel, RN ;
Thakker, GD ;
Lowenstein, CJ ;
Attur, MG ;
Abramson, SB .
FEBS LETTERS, 1997, 410 (2-3) :259-264
[4]  
Berman B, 2005, CUTIS, V75, P19
[5]   Anti-angiogenic effect of tetraacetyl-phytosphingosine [J].
Bin Kwon, Yoo ;
Kim, Chang Deok ;
Kim, Bo Joong ;
Kim, Min-Young ;
Park, Chang Seo ;
Yoon, Tae-Jin ;
Seo, Young-Joon ;
Suhr, Ki-Beom ;
Park, Jang-Kyu ;
Lee, Jeung-Hoon .
EXPERIMENTAL DERMATOLOGY, 2007, 16 (04) :311-317
[6]   Clinical and biochemical results of the metalloproteinase inhibition with subantimicrobial doses of doxycycline to prevent acute coronary syndromes (MIDAS) pilot trial [J].
Brown, DL ;
Desai, KK ;
Vakili, BA ;
Nouneh, C ;
Lee, HM ;
Golub, LM .
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 2004, 24 (04) :733-738
[7]  
BURNS FR, 1989, INVEST OPHTH VIS SCI, V30, P1569
[8]   Effects of sub-antimicrobial dose doxycycline therapy on crevicular fluid MMP-8, and gingival tissue MMP-9, TIMP-1 and IL-6 levels in chronic periodontitis [J].
Choi, DH ;
Moon, IS ;
Choi, BK ;
Paik, JW ;
Kim, YS ;
Choi, SH ;
Kim, CK .
JOURNAL OF PERIODONTAL RESEARCH, 2004, 39 (01) :20-26
[9]   AN EVALUATION OF MINOCYCLINE IN PATIENTS WITH PERIODONTAL-DISEASE [J].
CIANCIO, SG ;
MATHER, ML ;
MCMULLEN, JA .
JOURNAL OF PERIODONTOLOGY, 1980, 51 (09) :530-534
[10]   Nucleotide receptors: an emerging family of regulatory molecules in blood cells [J].
Di Virgilio, F ;
Chiozzi, P ;
Ferrari, D ;
Falzoni, S ;
Sanz, JM ;
Morelli, A ;
Torboli, M ;
Bolognesi, G ;
Baricordi, OR .
BLOOD, 2001, 97 (03) :587-600