Understanding the cellulolytic system of Trichoderma harzianum P49P11 and enhancing saccharification of pretreated sugarcane bagasse by supplementation with pectinase and α-L-arabinofuranosidase

被引：67

作者：

Delabona, Priscila da Silva ^{[1
]}

Cota, Junio ^{[1
]}

Hoffmam, Zaira Bruna ^{[1
]}

Alvaredo Paixao, Douglas Antonio ^{[1
]}

Farinas, Cristiane Sanchez ^{[2
]}

Lourenco Franco Cairo, Joao Paulo ^{[1
]}

Lima, Deise Juliana ^{[1
]}

Squina, Fabio Marcio ^{[1
]}

Ruller, Roberto ^{[1
]}

da Cruz Pradella, Jose Geraldo ^{[1
]}

机构：

[1] Brazilian Bioethanol Sci & Technol Lab CTBE, BR-13083970 Campinas, SP, Brazil

[2] Embrapa Instrumentat, BR-13560970 Sao Carlos, SP, Brazil

来源：

BIORESOURCE TECHNOLOGY | 2013年 / 131卷

基金：

巴西圣保罗研究基金会;

关键词：

Accessory enzymes; Trichoderma harzianum; Pectinase; alpha-L-arabinofuranosidase; Enzymatic hydrolysis; CELLULASE; HEMICELLULOSES; ENZYMES; FOREST;

D O I：

10.1016/j.biortech.2012.12.105

中图分类号：

S2 [农业工程];

学科分类号：

082806 [农业信息与电气工程];

摘要：

Supplementation of cellulase cocktails with accessory enzymes can contribute to a higher hydrolytic capacity in releasing fermentable sugars from plant biomass. This study investigated which enzymes were complementary to the enzyme set of Trichoderma harzianum in the degradation of sugarcane bagasse. Specific activities of T. harzianum extract on different substrates were compared with the extracts of Penicillium echinulatum and Trichoderma reesei, and two commercial cellulase preparations. Complementary analysis of the secretome of T. harzianum was also used to identify which enzymes were produced during growth on pretreated sugarcane bagasse. These analyses enabled the selection of the enzymes pectinase and alpha-L-arabinofuranosidase (AF) to be further investigated as supplements to the T. harzianum extract. The effect of enzyme supplementation on the efficiency of sugarcane bagasse saccharification was evaluated using response surface methodology. The supplementation of T. harzianum enzymatic extract with pectinase and AF increased the efficiency of hydrolysis by up to 116%. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：500 / 507

页数：8

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