Quantitative competitive polymerase chain reaction: Analysis of amplified products of the HIV-1 gag gene by capillary electrophoresis with laser-induced fluorescence detection

被引：23

作者：

Williams, SJ

Schwer, C

Krishnarao, ASM

Heid, C

Karger, BL

Williams, PM

机构：

[1] GENENTECH INC,DEPT ANALYT CHEM,S SAN FRANCISCO,CA 94080

[2] GENENTECH INC,DEPT BIOANALYT TECHNOL,S SAN FRANCISCO,CA 94080

[3] NORTHWESTERN UNIV,BARNETT INST,BOSTON,MA 02115

来源：

ANALYTICAL BIOCHEMISTRY | 1996年 / 236卷 / 01期

关键词：

D O I：

10.1006/abio.1996.0143

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A quantitative competitive polymerase chain reaction (PCR) assay for the detection and quantification of HIV-1 has been developed using capillary electrophoresis. Separation of the PCR products is carried out in coated capillaries filled with replaceable linear polyacrylamide, and detection is performed using laser-induced fluorescence. The quantitative capabilities of this assay are described. We show results from analysis of a noninfectious plasmid encoding the HIV genome and integrated proviral DNA. Potential applications of this assay are discussed. (C) 1996 Academic Press, Inc.

引用

页码：146 / 152

页数：7

共 30 条

[1] ENZYMATIC GENE AMPLIFICATION - QUALITATIVE AND QUANTITATIVE METHODS FOR DETECTING PROVIRAL DNA AMPLIFIED INVITRO [J].