Hydrolysis of a chitosan-induced milk aggregate by pepsin, trypsin and pancreatic lipase

被引:20
作者
Ausar, SF [1 ]
Landa, CA [1 ]
Bianco, ID [1 ]
Castagna, LF [1 ]
Beltramo, DM [1 ]
机构
[1] Cordoba Sci Agcy, CEPROCOR, RA-5164 Cordoba, Argentina
关键词
chitosan; casein; milk fat; protease; lipase;
D O I
10.1271/bbb.65.2412
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The addition of chitosan to whole milk results in dose dependent destabilization and coagulation of the casein micelles and milk fat. The present study evaluates how the presence of chitosan could affect the hydrolysis of this chitosan-induced aggregate by different gastrointestinal proteases (pepsin and trypsin) and by pancreatic lipase. The chitosan-milk aggregate was hydrolyzed by pepsin and trypsin, as evaluated by the UV absorbance of TCA-soluble peptides and by urea-PAGE. The kinetics and extent of hydrolysis were independent of the casein being soluble or aggregated. The release of soluble peptides from the aggregate was independent of the presence of chitosan. A progressive inhibition of pancreatic lipase was observed in proportion to the increase in molecular weight of the chitosan employed to induce the formation of the aggregate. Interestingly, the presence of chitosan not only affected the initial velocity of the reaction, but also reduced its extent. The results indicate that a milk aggregate induced by chitosan was very well digested by gastric and intestinal proteases independently of the molecular weight of the chitosan used, and that the aggregate could retain the lipid-lowering effect of chitosan.
引用
收藏
页码:2412 / 2418
页数:7
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