Red pool chlorophylls of photosystem I of the cyanobacterium Thermosynechococcus elongatus:: A single-molecule study

被引：29

作者：

Elli, AF

Jelezko, F

Tietz, C

Studier, H

Brecht, M

Bittl, R

Wrachtrup, J

机构：

[1] Univ Stuttgart, Inst Phys, D-70569 Stuttgart, Germany

[2] Free Univ Berlin, Fachbereich Phys, D-14195 Berlin, Germany

来源：

BIOCHEMISTRY | 2006年 / 45卷 / 05期

关键词：

D O I：

10.1021/bi0521700

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Photosystem I reaction centers of the cyanobacterium Thermosynechococcus elongatus have been investigated using single-molecule spectroscopy. Single-molecule fluorescence emission spectra reveal a new fluorescence band located at 745 nm. Fluorescence polarization spectroscopy and fluorescence autocorrelation analysis show that only a few chlorophylls are responsible for the photoemission from the Photosystem I trimer at low temperature. Intersystem crossing parameters of the red pool chlorophylls have been determined via fluorescence autocorrelation measurements. The triplet yield of the red chlorophylls is strongly reduced in comparison to chlorophyll a in solution. Strong quenching of the triplet state indicates that the red chlorophylls are located in close contact to carotenoids.

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页码：1454 / 1458

页数：5

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