Isolation and Characterization of Polymorphic Microsatellite Markers from the Chinese Medicinal Herb Atractylodes macrocephala (Asteraceae)

被引：14

作者：

Zheng, Li ^{[1
,2
,3
]}

Shao, Zhong-Da ^{[1
,2
,3
]}

Wang, Zong-Chao ^{[1
,2
,3
]}

Fu, Cheng-Xin ^{[1
,2
,3
]}

机构：

[1] Zhejiang Univ, Coll Life Sci, Minist Educ, Key Lab Conservat Biol Endangered Wildlife, Hangzhou 310058, Zhejiang, Peoples R China

[2] Zhejiang Univ, Inst Plant Sci, Lab Systemat & Evolutionary Bot & Biodivers, Hangzhou 310058, Zhejiang, Peoples R China

[3] Zhejiang Univ, Conservat Ctr Gene Resources Endangered Wildlife, Hangzhou 310058, Zhejiang, Peoples R China

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2012年 / 13卷 / 12期

基金：

中国国家自然科学基金;

关键词：

Atractylodes macrocephala; microsatellites; genetic diversity; Chinese medicinal herb; ATRACTYLENOLIDE-III; OVATA;

D O I：

10.3390/ijms131216046

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Atractylodes macrocephala Koidz. (Asteraceae) is an economically important Chinese medicinal herb. In this study, 15 polymorphic microsatellite markers were developed from A. macrocephala using the compound microsatellite marker technique. Levels of polymorphism within the 15 markers were assessed using 83 individuals from two wild and two cultivated populations in China. The number of alleles per locus ranged from 2 to 20, with an average of 9.9 alleles. Observed and expected heterozygosities ranged from 0.083 to 1.000 and from 0.097 to 0.938, respectively. These markers will be valuable for germplasm classification and identification, as well as for assessing the genetic diversity and spatial genetic structure among wild and cultivated populations of A. macrocephala.

引用

页码：16046 / 16052

页数：7

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