Analysis of several iridoid and indole precursors of terpenoid indole alkaloids with a single HPLC run

被引:21
作者
Dagnino, D
Schripsema, J
Verpoorte, R
机构
[1] TECH UNIV DENMARK,DEPT ORGAN CHEM,DK-2800 LYNGBY,DENMARK
[2] LEIDEN UNIV,CTR BIOPHARMACEUT SCI,DIV PHARMACOGNOSY,NL-2300 RA LEIDEN,NETHERLANDS
关键词
D O I
10.1055/s-2006-957879
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
An isocratic HPLC system is described which allows the separation of the iridoid and indole precursors of terpenoid indole alkaloids, which are present in a single crude extract. The system consists of a column of LiChrospher 60 RP select 6 5 mu m, 250 x 4mm (Merck) with an eluent of 1 % formic acid-acetonitrile-trichloroacetic acid (100:10:0.25, v:v:w) at a flow of 1.2 ml/min. In the suspension cultures of Catharanthus roseus secologanin and tryptophan were detected. In the cultures of Tabernaemontana divaricata loganin, tryptophan, and tryptamine accumulated.
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页码:278 / 280
页数:3
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