Suspension array technology: evolution of the flat-array paradigm

被引：301

作者：

Nolan, JP

Sklar, LA

机构：

[1] Los Alamos Natl Lab, Biosci Div, Los Alamos, NM 87545 USA

[2] Univ New Mexico, Hlth Sci Ctr, Natl Flow Cytometry Resource & Cytometry Canc Res, Albuquerque, NM 87131 USA

来源：

TRENDS IN BIOTECHNOLOGY | 2002年 / 20卷 / 01期

关键词：

D O I：

10.1016/S0167-7799(01)01844-3

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Suspension arrays of microspheres analyzed using flow cytometry offer a new approach to multiplexed assays for large-scale screening applications. By optically encoding micron-sized polymer particles, suspension microarrays can be created to enable highly multiplexed analysis of complex samples. Each element in the array is comprised of a subpopulation of particles with distinct optical properties and each array element bears a different surface receptor. Nucleic acids, proteins, lipids or carbohydrates can serve as receptors to support the analysis of a wide range of biomolecular assemblies, and applications in genomic and proteomic research are being developed. Coupled with recent innovations for rapid serial analysis of samples, molecular analysis with microsphere arrays holds significant potential as a general analysis platform for both research and clinical applications.

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页码：9 / 12

页数：4

相关论文

共 32 条

[1] Optimal screening of surface-displayed polypeptide libraries [J].

Boder, ET ;

Wittrup, KD .

BIOTECHNOLOGY PROGRESS, 1998, 14 (01) :55-62

[2] Gene expression analysis by massively parallel signature sequencing (MPSS) on microbead arrays [J].

Brenner, S ;

Johnson, M ;

Bridgham, J ;

Golda, G ;

Lloyd, DH ;

Johnson, D ;

Luo, SJ ;

McCurdy, S ;

Foy, M ;

Ewan, M ;

Roth, R ;

George, D ;

Eletr, S ;

Albrecht, G ;

Vermaas, E ;

Williams, SR ;

Moon, K ;

Burcham, T ;

Pallas, M ;

DuBridge, RB ;

Kirchner, J ;

Fearon, K ;

Mao, J ;

Corcoran, K .

NATURE BIOTECHNOLOGY, 2000, 18 (06) :630-634

[3] Flow cytometry-based minisequencing: A new platform for high-throughput single-nucleotide polymorphism scoring [J].

Cai, H ;

White, PS ;

Torney, D ;

Deshpande, A ;

Wang, ZL ;

Marrone, B ;

Nolan, JP .

GENOMICS, 2000, 66 (02) :135-143

[4] SNPing in the human genome [J].

Carlson, CS ;

Newman, TL ;

Nickerson, DA .

CURRENT OPINION IN CHEMICAL BIOLOGY, 2001, 5 (01) :78-85

[5] Simultaneous quantitation of 15 cytokines using a multiplexed flow cytometric assay [J].

Carson, RT ;

Vignali, DAA .

JOURNAL OF IMMUNOLOGICAL METHODS, 1999, 227 (1-2) :41-52

[6] A microsphere-based assay for multiplexed single nucleotide polymorphism analysis using single base chain extension [J].

Chen, JW ;

Iannone, MA ;

Li, MS ;

Taylor, JD ;

Rivers, P ;

Nelsen, AJ ;

Slentz-Kesler, KA ;

Roses, A ;

Weiner, MP .

GENOME RESEARCH, 2000, 10 (04) :549-557

[7]

Chen R, 1999, CLIN CHEM, V45, P1693

[8] Flow cytometric screening of cell-based libraries [J].

Daugherty, PS ;

Iverson, BL ;

Georgiou, G .

JOURNAL OF IMMUNOLOGICAL METHODS, 2000, 243 (1-2) :211-227

[9] 11-color, 13-parameter flow cytometry: Identification of human naive T cells by phenotype, function, and T-cell receptor diversity [J].

De Rosa, SC ;

Herzenberg, LA ;

Herzenberg, LA ;

Roederer, M .

NATURE MEDICINE, 2001, 7 (02) :245-248

[10] HTPS flow cytometry: A novel platform for automated high throughput drug discovery and characterization [J].

Edwards, BS ;

Kuckuck, FW ;

Prossnitz, ER ;

Ransom, JT ;

Sklar, LA .

JOURNAL OF BIOMOLECULAR SCREENING, 2001, 6 (02) :83-90

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