Isolation of an extremely acidophilic and highly efficient strain Acidithiobacillus sp for chalcopyrite bioleaching

被引:31
作者
Feng, Shoushuai [1 ]
Yang, Hailin [1 ]
Xin, Yu [1 ]
Zhang, Ling [1 ]
Kang, Wenliang [1 ]
Wang, Wu [1 ]
机构
[1] Jiangnan Univ, Sch Biotechnol, Minist Educ, Key Lab Ind Biotechnol, Wuxi 214122, Peoples R China
基金
国家高技术研究发展计划(863计划);
关键词
Acidithiobacillus; Extremophile; Jarosite; Chalcopyrite; Highly efficient bioleaching; THIOBACILLUS-FERROOXIDANS; MICROBIAL COMMUNITIES; IRON OXIDATION; CLONE LIBRARY; HEAP; PROGRESS; METALS; PART; NOV;
D O I
10.1007/s10295-012-1174-1
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
An extremely acidophilic sulfur-oxidizing bacterium was isolated from an industrial-scale bioheap of the Zijinshan copper mine and was named ZJJN. A tuft of flagella and a layer of thick capsule outside the cell envelope were clearly observed under transmission electron microscopy (TEM), which might be closely related to the extremely acid-proof capacity of ZJJN cells in the bioleaching system; 16S ribosomal RNA (rRNA) phylogeny showed that the isolated strain was highly homologous to the genera of Acidithiobacillus. The optimum temperature of ZJJN was determined at 30 A degrees C and pH at 1.0. It was capable of growth at even pH 0. Strain ZJJN can utilize reduced sulfur as an energy source but not with organics or ferrous ion. Strain ZJJN was sensitive to all antibiotics with different concentrations; when it showed a certain resistance to different concentrations of Cu2+. In the mixed strains of ZJJN and A. ferrooxidans system (initial pH 1.0), the copper-leaching efficiency was up to 60.1 %, which was far higher than other systems. Scanning electron microscopy (SEM) analysis showed that less jarosite precipitation was produced in the most efficient system. The extremely acidophilic strain ZJJN would be of great potential in the application of chalcopyrite bioleaching.
引用
收藏
页码:1625 / 1635
页数:11
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