Matrix-assisted laser desorption ionisation-time-of of-flight mass spectrometry of intact cells allows rapid identification of Burkholderia cepacia complex

被引：68

作者：

Vanlaere, Elke ^{[1
]}

Sergeant, Kjell ^{[2
]}

Dawyndt, Peter ^{[1
]}

Kallow, Wibke ^{[3
]}

Erhard, Marcel ^{[3
]}

Sutton, Helen ^{[4
]}

Dare, Diane ^{[2
,4
]}

Devreese, Bart ^{[2
]}

Samyn, Bart ^{[2
]}

Vandamme, Peter ^{[1
]}

机构：

[1] Univ Ghent, Microbiol Lab, B-9000 Ghent, Belgium

[2] Univ Ghent, Lab Eiwitbiochem Eiwitengn, B-9000 Ghent, Belgium

[3] Gesell Analyt Biochem & Diagnost mbH, AnagnosTec, D-14476 Potsdam, Germany

[4] Manchester Metropolitan Univ, Runcorn WA7 4QX, Cheshire, England

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2008年 / 75卷 / 02期

关键词：

Burkholderia cepacia complex; Cystic fibrosis; Identification; MALDI-TOF MS;

D O I：

10.1016/j.mimet.2008.06.016

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The present study examined the potential of intact-cell matrix-assisted laser desorption ionisation-time-of-flight mass spectrometry (MALDI-TOF MS) for a rapid identification of Burkholderia cepacia complex (Bcc) bacteria using an Applied Biosystems 4700 Proteomics Analyser. Two software packages were used to analyse mass profiles based on densitometric curves and peak positions. The 75 strains examined, represented the nine established Bcc species and some commonly misidentified species, closely related or biochemically similar to Bcc and relevant in the context of cystic fibrosis microbiology. All Bcc strains clustered together, separated from non-Bcc strains. Within Bcc, most Bcc strains grouped in species specific clusters, except for Burkholderia anthina and Burkholderia pyrrocinia strains which constituted a single cluster. The present study demonstrates that MALDI-TOF MS is a powerful approach for the rapid identification of Bcc bacteria. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：279 / 286

页数：8

共 35 条

[1] Multilocus sequence typing scheme that provides both species and strain differentiation for the Burkholderia cepacia complex [J].