Zn2+ dependent DNA binders based on terminally modified peptide nucleic acids

被引：4

作者：

Boll, I ^{[1
]}

Kovbasyuk, L ^{[1
]}

Krämer, R ^{[1
]}

Oeser, T ^{[1
]}

Mokhir, A ^{[1
]}

机构：

[1] Heidelberg Univ, D-69120 Heidelberg, Germany

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2006年 / 16卷 / 10期

关键词：

peptide nucleic acids; zinc; DNA; binding;

D O I：

10.1016/j.bmcl.2006.02.002

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Two ligand-intercalator-peptide nucleic acid conjugates (L-NADI-PNAs) have been synthesized. Affinity of these conjugates to their complementary DNAs was found to be affected by Zn2+. The magnitude of this effect Could be controlled by a variation of the ligand. Upon binding Zn2+ the L-NADI-PNAs form positively charged ZnL complexes, which interact 2 with the negatively charged DNA backbone. This electrostatic interaction stabilizes PNA/DNA duplexes. It has been found that Zn2+ dependent stabilization takes place only if the ZnL complex has a higher total positive charge than the ligand. Linear correlation has been observed between Zn2+ induced stabilization of PNA/DNA duplexes and difference of charges of the ZnL complex and the ligand. (C) 2006 Elsevier Ltd. All rights reserved.

引用

页码：2781 / 2785

页数：5

共 20 条

[1] Zinc metabolism in the brain: Relevance to human neurodegenerative disorders [J].