Twin probes as a novel tool for the detection of single-nucleotide polymorphisms

被引：26

作者：

Ergen, Erhan ^{[1
]}

Weber, Markus ^{[1
]}

Jacob, Josemon ^{[1
]}

Herrmann, Andreas ^{[1
]}

Muellen, Klaus ^{[1
]}

机构：

[1] Max Planck Inst Polymer Res, D-55128 Mainz, Germany

来源：

CHEMISTRY-A EUROPEAN JOURNAL | 2006年 / 12卷 / 14期

关键词：

DNA detection; DNA; fluorescent probes; hybridization;

D O I：

10.1002/chem.200501526

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Single-nucleotide polymorphisms (SNPs) are the most common form of DNA sequence variation. There is a strong interest from both academy and industry to develop rapid, sensitive and cost effective methods for SNP detection. Here we report a novel structural concept for DNA detection based on fluorescence dequenching upon hybridization. The so-called "twin probe" consists of a central fluorene derivative as fluorophore to which two identical oligonucleotides are covalently attached. This probe architecture is applied in homogeneous hybridization assays with subsequent fluorescence spectroscopic analysis. The bioorganic hybrid structure is well suited for sequence specific DNA detection and even SNPs are identified with high efficiency. Additionally, the photophysical properties of the twin probe were investigated. The covalent attachment of two single stranded oligonucleotides leads to strong quenching of the central fluorescence dye induced by the nucleobases. The twin probe is characterized by supramolecular aggregate formation accompanied by red-shifted emission and broad fluorescence spectra.

引用

页码：3707 / 3713

页数：7

共 33 条

[1] Thermodynamic basis of the enhanced specificity of structured DNA probes [J].