Neurodegenerative and neuroprotective effects of tumor necrosis factor (TNF) in retinal ischemia: Opposite roles of TNF receptor 1 and TNF receptor 2

被引:381
作者
Fontaine, V
Mohand-Said, S
Hanoteau, N
Fuchs, L
Pfizenmaier, K
Eisel, U
机构
[1] Univ Stuttgart, Inst Cell Biol & Immunol, D-70569 Stuttgart, Germany
[2] INSERM, Lab Physiopathol Cellulaire & Mol Retina, F-67091 Strasbourg, France
关键词
Akt/PKB activation; neuronal TNF and TNF-R expression; retinal cell layers; ischemia; neuroprotection; knock-out mouse; immunohistology;
D O I
10.1523/JNEUROSCI.22-07-j0001.2002
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Tumor necrosis factor (TNF) is an important factor in various acute and chronic neurodegenerative disorders. In retinal ischemia, we show early, transient upregulation of TNF, TNF receptor 1 (TNF-R1), and TNF-R2 6 hr after reperfusion preceding neuronal cell loss. To assess the specific role of TNF and its receptors, we compared ischemia-reperfusion- induced retinal damage in mice deficient for TNF-R1, TNF-R2, or TNF by quantifying neuronal cell loss 8 d after the insult. Surprisingly, TNF deficiency did not affect overall cell loss, yet absence of TNF-R1 led to a strong reduction of neurodegeneration and lack of TNF-R2 led to an enhancement of neurodegeneration, indicative of TNF-independent and TNF-dependent processes in the retina, with TNF-R1 augmenting neuronal death and TNF-R2 promoting neuroprotection. Western blot analyses of retinas revealed that reduction of neuronal cell loss in TNFR1 -/- animals correlated with the presence of activated Akt/ protein kinase B (PKB). Inhibition of the phosphatidylinositol 3-kinase signaling pathway reverted neuroprotection in TNFR1-deficient mice, indicating an instrumental role of Akt/ PKB in neuroprotection and TNF-R2 dependence of this pathway. Selective inhibition of TNF-R1 function may represent a new approach to reduce ischemia-induced neuronal damage, being potentially superior to strategies aimed at suppression of TNF activity in general.
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