Screening of rhizobacteria isolated from maize (Zea mays L.) in Rio Grande do Sul State (South Brazil) and analysis of their potential to improve plant growth

被引:85
作者
Arruda, Leticia [1 ]
Beneduzi, Anelise [2 ]
Martins, Adriana [3 ]
Lisboa, Bruno [2 ]
Lopes, Cristiane [2 ]
Bertolo, Fernanda [2 ]
Passaglia, Luciane Maria P. [1 ]
Vargas, Luciano K. [2 ]
机构
[1] Univ Fed Rio Grande do Sul, Inst Biociencias, Dept Genet, BR-91501970 Porto Alegre, RS, Brazil
[2] Fundacao Estadual Pesquisa Agr FEPAGRO, BR-90130060 Porto Alegre, RS, Brazil
[3] Univ Fed Paraiba, Dept Solos & Engn Rural, Ctr Ciencias Agr, Areia, PB, Brazil
关键词
Maize; PGPR; IAA; Bacterial diversity; Siderophore; Phosphate solubilization; AZOSPIRILLUM-BRASILENSE; PROMOTING RHIZOBACTERIA; SOIL; RHIZOSPHERE; PSEUDOMONAS; BACTERIA; STRAINS; SOLUBILIZATION; BIOSYNTHESIS; SIDEROPHORES;
D O I
10.1016/j.apsoil.2012.09.001
中图分类号
S15 [土壤学];
学科分类号
0903 ; 090301 ;
摘要
Plant growth-promoting rhizobacteria (FUR) are considered to have a beneficial effect on host plants and may facilitate plant growth by different mechanisms. In this work, the influence of different soil types on the bacterial diversity and the stimulatory effects of selected PGPR on two cultivars of maize were investigated. A set of 292 strains was isolated from the roots and rhizosphere soil of maize cultivated in five different areas of the Rio Grande do Sul State in Brazil. 16S rDNA-PCR-RFLP and 16S rDNA partial sequencing were used for identification, and the Shannon-Weaver index was used to evaluate bacterial diversity. We evaluated the ability of each isolate to produce indole acetic acid (IAA), siderophores and solubilize phosphates. On the basis of multiple PGP traits, six isolates were selected to test their potential as plant growth-promoting rhizobacteria on maize plants. In both the roots and the rhizospheric soil of maize, the dominant bacterial genera identified were Klebsiella and Burkholderia. IAA producers were distributed widely among isolates, regardless of the sampling site. Approximately 42% of the isolates exhibited at least two attributes, and 24% showed all three PGP traits. Three strains, identified as Achromobacter, Burkholderia, and Arthrobacter, were effective as PGPR in both of the cultivars evaluated. (C) 2012 Elsevier B.V. All rights reserved.
引用
收藏
页码:15 / 22
页数:8
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