Sequence analysis of 16S rRNA genes amplified from two ribosomal RNA gene clusters of Bifidobacterium bifidum

被引：7

作者：

Yoon, JH

Yim, DK

Goodfellow, M

Park, YH

机构：

[1] Korea Res Inst Biosci & Biotechnol, Korean Collect Type Cultures, Taejon 305600, South Korea

[2] Univ Newcastle Upon Tyne, Dept Agr & Environm Sci, Newcastle Upon Tyne NE1 7RU, Tyne & Wear, England

来源：

ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY | 1999年 / 75卷 / 04期

关键词：

Bifidobacterium bifidum; rRNA operon; 16S rDNA;

D O I：

10.1023/A:1002025100444

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Two rRNA gene clusters were detected in the genome of Bifidobacterium bifidum KCTC 3202(T) using Southern blot analysis. To analyse the sequences of the 16S rRNA genes from rrnA and rrnB, 16S rDNAs were amplified by PCR using DNA fragments purified from gel slices containing each of the rRNA gene clusters. The amplified 16S rDNAs from rrnA and rrnB were cloned into vectors and three clones of each gene sequenced. The resultant sequences were confirmed by direct sequencing of the 16S rDNAs from rrnA and rrnB. Sequence differences were not found between rrnA and rrnB in 1488 bp of the 16S rRNA genes.

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页码：329 / 333

页数：5

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