DNAPROBE, a computer program which generates oligonucleotide probes from protein alignments

被引：1

作者：

Drummond, M ^{[1
]}

Stamper, J ^{[1
]}

机构：

[1] John Innes Inst, Dept Mol Microbiol, Nitrogen Fixat Lab, Norwich NR4 7UH, Norfolk, England

来源：

NUCLEIC ACIDS RESEARCH | 1999年 / 27卷 / 17期

关键词：

D O I：

10.1093/nar/27.17.3493

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe a program to assist in designing oligonucleotide probes on the basis of protein alignments and the codon usage of the target organism. If necessary, the input sequences can be weighted to neutralise the effect of closely similar sequences or to bias the output in favour of a particular taxon.

引用

页码：3493 / 3493

页数：1

共 6 条

[1] IMPROVED FREE-ENERGY PARAMETERS FOR PREDICTIONS OF RNA DUPLEX STABILITY [J].

FREIER, SM ;

KIERZEK, R ;

JAEGER, JA ;

SUGIMOTO, N ;

CARUTHERS, MH ;

NEILSON, T ;

TURNER, DH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (24) :9373-9377

[2] VOLUME CHANGES IN PROTEIN EVOLUTION [J].

GERSTEIN, M ;

SONNHAMMER, ELL ;

CHOTHIA, C .

JOURNAL OF MOLECULAR BIOLOGY, 1994, 236 (04) :1067-1078

[3] A COMPUTER-PROGRAM FOR SELECTION OF OLIGONUCLEOTIDE PRIMERS FOR POLYMERASE CHAIN REACTIONS [J].

LOWE, T ;

SHAREFKIN, J ;

YANG, SQ ;

DIEFFENBACH, CW .

NUCLEIC ACIDS RESEARCH, 1990, 18 (07) :1757-1761

[4]

NASH JHE, 1993, COMPUT APPL BIOSCI, V9, P469

[5]

RAJKUMAR VP, 1990, NUCLEIC AIDS RES, V18, P3080

[6] A COMPUTER-PROGRAM FOR CHOOSING OPTIMAL OLIGONUCLEOTIDES FOR FILTER HYBRIDIZATION, SEQUENCING AND INVITRO AMPLIFICATION OF DNA [J].

RYCHLIK, W ;

RHOADS, RE .

NUCLEIC ACIDS RESEARCH, 1989, 17 (21) :8543-8551

← 1 →