Treatment of soy milk with Debaryomyces hansenii cells immobilised in alginate

被引：9

作者：

de Souza Junior, Waldeck Campanha ^{[1
]}

de Rezende, Sebastiao Tavares ^{[1
]}

Viana, Pollyanna Amaral ^{[1
]}

Falkoski, Daniel Luciano ^{[1
]}

Reis, Angelica Pataro ^{[1
]}

Machado, Solimar Goncalves ^{[1
]}

de Barros, Everaldo Goncalves ^{[1
]}

Guimaraes, Valeria Monteze ^{[1
]}

机构：

[1] Univ Fed Vicosa, BIOAGRO, Dept Bioquim & Biol Mol, BR-36570000 Vicosa, MG, Brazil

来源：

FOOD CHEMISTRY | 2009年 / 114卷 / 02期

关键词：

Calcium alginate; Cell immobilization; Debaryomyces hansenii; UFV-1; alpha-Galactosidase; Galactooligosaccharides; ALPHA-GALACTOSIDASE; SOYBEAN PRODUCTS; HYDROLYSIS; OLIGOSACCHARIDES; SOYMILK; LACTOSE; UFV-1;

D O I：

10.1016/j.foodchem.2008.09.090

中图分类号：

O69 [应用化学];

学科分类号：

070301 [无机化学];

摘要：

Whole cells of Debaryomyces hansenii UFV-1 were permeabilised with ethanol and immobilised in calcium alginate hydrogel. The optimum pH and temperature for alpha-galactosidase activities of permeabilised free (PFC) and permeabilised immobilised cells (PIC) were 4.5 and 60 degrees C; and 4.0 and 70 degrees C, respectively. PIC alpha-galactosidase was more stable than that of PFC. The incubation of PIC at 60 and 70 degrees C promoted an increase in alpha-galactosidase activity. The alpha-galactosidase activity was maintained when PIC was used in three repeated batches. The K-m values for PIC and PFC alpha-galactosidases, with pNP alpha GaL were 0.82 mM and 0.30 mM, respectively. Soy milk treatment with PIC for 6 h at 60 degrees C promoted 100% hydrolysis of raffinose oligosaccharides. (c) 2008 Elsevier Ltd. All rights reserved.

引用

页码：589 / 593

页数：5

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