Sitosterol-β-glucoside as primer for cellulose synthesis in plants

被引：302

作者：

Peng, LC ^{[1
]}

Kawagoe, Y ^{[1
]}

Hogan, P ^{[1
]}

Delmer, D ^{[1
]}

机构：

[1] Univ Calif Davis, Plant Biol Sect, Davis, CA 95616 USA

来源：

SCIENCE | 2002年 / 295卷 / 5552期

关键词：

D O I：

10.1126/science.1064281

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Cellulose synthesis in plants requires beta -1,4-glucan chain initiation, elongation, and termination. The process of chain elongation is likely to be distinct from the process of chain initiation. We demonstrate that a CesA glucosyltransferase initiates glucan polymerization by using sitosterol-beta -glucoside (SG) as primer. Cotton fiber membranes synthesize sitosterol-cellodextrins (SCDs) from SG and uridine 5'-diphosphate-glucose (UDP-Glc) under conditions that also favor cellulose synthesis. The cellulase encoded by the Korrigan (Kor) gene, required for cellulose synthesis in plants, may function to cleave SG from the growing polymer chain.

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页码：147 / 150

页数：4

相关论文

共 25 条

[1] A SIMPLE AND RAPID PREPARATION OF ALDITOL ACETATES FOR MONOSACCHARIDE ANALYSIS [J].

BLAKENEY, AB ;

HARRIS, PJ ;

HENRY, RJ ;

STONE, BA .

CARBOHYDRATE RESEARCH, 1983, 113 (02) :291-299

[2] IN-VITRO SYNTHESIS OF A MICROFIBRILLAR (1-]3)-BETA-GLUCAN BY A RYEGRASS (LOLIUM-MULTIFLORUM) ENDOSPERM (1-]3)-BETA-GLUCAN SYNTHASE ENRICHED BY PRODUCT ENTRAPMENT [J].

BULONE, V ;

FINCHER, GB ;

STONE, BA .

PLANT JOURNAL, 1995, 8 (02) :213-225

[3] BETA-GLUCOSIDE ACTIVATORS OF MUNG BEAN UDP-GLUCOSE - BETA-GLUCAN SYNTHASE .2. COMPARISON OF EFFECTS OF AN ENDOGENOUS BETA-LINKED GLUCOLIPID WITH SYNTHETIC NORMAL-ALKYL BETA-D-MONOGLUCOPYRANOSIDES [J].

CALLAGHAN, T ;

ROSS, P ;

WEINBERGEROHANA, P ;

BENZIMAN, M .

PLANT PHYSIOLOGY, 1988, 86 (04) :1104-1107

[4]

Cantatore JL, 2000, BIOCHEM SOC T, V28, P748, DOI 10.1042/BST0280748

[5] Molecular directionality of polysaccharide polymerization by the Pasteurella multocida hyaluronan synthase [J].

DeAngelis, PL .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (37) :26557-26562

[6] Cellulose biosynthesis: Exciting times for a difficult field of study [J].

Delmer, DP .

ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 1999, 50 :245-276

[7] BIOSYNTHESIS AND STRUCTURE OF GLYCOSYL DIGLYCERIDES, STERYL GLUCOSIDES, AND ACYLATED STERYL GLUCOSIDES [J].

ELBEIN, AD ;

FORSEE, WT ;

SCHULTZ, JC ;

LAINE, RA .

LIPIDS, 1975, 10 (07) :427-436

[8] PROCUSTE1 encodes a cellulose synthase required for normal cell elongation specifically in roots and dark-grown hypocotyls of arabidopsis [J].

Fagard, M ;

Desnos, T ;

Desprez, T ;

Goubet, F ;

Refregier, G ;

Mouille, G ;

McCann, M ;

Rayon, C ;

Vernhettes, S ;

Höfte, H .

PLANT CELL, 2000, 12 (12) :2409-2423

[9]

FUJINO Y, 1979, BIOCHIM BIOPHYS ACTA, V574, P94

[10] Chitin oligosaccharide synthesis by rhizobia and zebrafish embryos starts by glycosyl transfer to O4 of the reducing-terminal residue [J].

Kamst, E ;

Bakkers, J ;

Quaedvlieg, NEM ;

Pilling, J ;

Kijne, JW ;

Lugtenberg, BJJ ;

Spaink, HP .

BIOCHEMISTRY, 1999, 38 (13) :4045-4052