Expressing a bacterial mercuric ion binding protein in plant for phytoremediation of heavy metals

被引：35

作者：

Hsieh, Ju-Liang ^{[1
]}

Chen, Ching-Yi ^{[1
]}

Chiu, Meng-Hsuen ^{[1
]}

Chein, Mei-fang ^{[2
]}

Chang, Jo-Shu ^{[3
,4
]}

Endo, Ginro ^{[2
]}

Huang, Chieh-Chen ^{[1
]}

机构：

[1] Natl Chung Hsing Univ, Dept Life Sci, Taichung 40227, Taiwan

[2] Tohoku Gakuin Univ, Fac Engn, Lab Environm Biotechnol, Tagajo, Miyagi 9858537, Japan

[3] Natl Cheng Kung Univ, Dept Chem Engn, Tainan 701, Taiwan

[4] Natl Cheng Kung Univ, Sustainable Environm Res Ctr, Tainan 70101, Taiwan

来源：

JOURNAL OF HAZARDOUS MATERIALS | 2009年 / 161卷 / 2-3期

关键词：

Biosorption; Gram-positive bacterial MerP; Heavy metals; Mercuric ion binding protein; Phytoremediation; CLASS-II TRANSPOSON; ORGANOMERCURIAL COMPOUNDS; RESISTANCE DETERMINANTS; GENES; TRANSFORMATION; AGROBACTERIUM; DIVERSITY; PEPTIDES; LYASE;

D O I：

10.1016/j.jhazmat.2008.04.079

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

A specific mercuric ion binding protein (MerP) originating from transposon TnMERI1 of Bacillus megaterium strain MB1 isolated from Minamata Bay displayed good adsorption capability for a variety of heavy metals. In this study, the Gram-positive MerP protein was expressed in transgenic Arabidopsis to create a model system for phytoremediation of heavy metals. Under control of an actin promoter, the transgenic Arabidpsis showed higher tolerance and accumulation capacity for mercury, cadium and lead when compared with the control plant. Results from confocal microscopy analysis also indicate that MerP was localized at the cell membrane and vesicles of plant cells. The developed transgenic plants possessing excellent meta1-accumulative ability could have potential applications in decontamination of heavy metals. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：920 / 925

页数：6

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