Effect of C-reactive protein on chemokine expression in human aortic endothelial cells

被引:94
作者
Devaraj, S
Kumaresan, PR
Jialal, I
机构
[1] Univ Calif Davis, Med Ctr, Dept Pathol, Lab Atherosclerosis & Metab Res, Sacramento, CA 95817 USA
[2] Univ Calif Davis, Med Ctr, Dept Internal Med, Sacramento, CA 95817 USA
关键词
inflammation; endothelium; chemokine; interleukin; C-reactive protein;
D O I
10.1016/j.yjmcc.2003.12.005
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Inflammation plays a pivotal role in atherosclerosis. In addition to being a risk marker for cardiovascular disease, much recent data support a role for C-reactive protein (CRP) in atherogenesis. Interleukin-8 (IL-8), a member of the CXC chemokines promotes monocyte-endothelial cell adhesion and arrest and is abundant in atherosclerotic plaques. However, there is a paucity of data examining the effect of CRP on IL-8 secretion in human aortic endothelial cells (HAEC). In this report, we show that incubation of HAEC with CRP resulted in a time and dose-dependent increase in IL-8 protein and mRNA via transcription. In contrast to human umbilical vein endothelial cells, monocyte-chemoattractant protein-1 expression in HAEC was not affected by CRR Furthermore, CRP upregulated NF-kappa B activity in HAEC and inhibitors of NF-kappa B significantly reversed the upregulation of IL-8 by CRP. Blocking antibodies to IL-8 significantly decreased monocyte-endothelial cell adhesion induced by CRP (31 %, P < 0.01). In conclusion, this study makes the novel observation that CRP induces IL-8 synthesis and secretion in HAEC via upregulation of NF-kappa B activity. (C) 2003 Elsevier Ltd. All rights reserved.
引用
收藏
页码:405 / 410
页数:6
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