Modulation of signalling nuclear factor-κB activation pathway by polyphenols in human intestinal Caco-2 cells

被引:151
作者
Romier, Beatrice
Van De Walle, Jacqueline
During, Alexandrine
Larondelle, Yvan
Schneider, Yves-Jacques [1 ]
机构
[1] Inst Sci, B-1348 Louvain, Belgium
关键词
intestinal inflammation; nuclear factor-kappa B; inhibitor of kappa B pathway; interleukin-8; production; polyphenols; Caco-2; cells;
D O I
10.1017/S0007114508966666
中图分类号
R15 [营养卫生、食品卫生]; TS201 [基础科学];
学科分类号
100403 ;
摘要
Recent Studies support beneficial effects of polyphenols in various chronic inflammatory diseases, for example, the inflammatory bowel diseases. Inhibition of NF-kappa B activation by polyphenols could explain part of their anti-inflammatory properties, but few data are available on the intestine. The purpose of the present Study was thus to investigate the effects of some polyphenols on NF-kappa B activation using human intestinal Caco-2 cells. Effects of standard polyphenols (50 mu mol/l) were studied on different cellular events associated with NF-kappa B activation: (i) NF-kappa B activity using cells transiently transfected with a NF-kappa B-luciferase construct and stimulated by inflammatory agents (IL-1 beta, TNF-alpha or lipopolysaccharides (LPS)); (ii) phosphorylation of the inhibitor of kappa B (I kappa B-alpha) analysed by Western blot; (iii) secretion of IL-8 quantified by ELISA assay. Results showed that chrysin and ellagic acid inhibited NF-kappa B activity, whereas genistein and resveratrol increased it. These effects were independent of the nature of the inducer, indicating that polyphenols may modulate NF-kappa B activation by acting on a common event to the cytokine- and LPS-mediated cascades. Chrysin strongly reduced (2.5-fold) IL-1 beta-induced I kappa B-alpha phosphorylation, whereas ellagic acid increased it (1.7-fold). Ellagic acid, genistein and epigallocatechin.-allate reduced (4- to 8-fold) IL-1 beta-induced IL-8 secretion, while resveratrol promoted (1.7-fold) the secretion. Chrysin also diminished IL-8 secretion by 1.6-fold (but P > 0.05). The data indicate that polyphenols can modulate the NF-kappa B activation pathway in the intestine. Chrysin could block NF-kappa B activation via the inhibition of I kappa B-alpha phosphorylation. The other molecular targets of the active polyphenols are still to be identified.
引用
收藏
页码:542 / 551
页数:10
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