Enrichment and isolation of iron-oxidizing bacteria at neutral pH

被引：130

作者：

Emerson, D ^{[1
]}

Floyd, MM

机构：

[1] Amer Type Culture Collect, Manassas, VA 20110 USA

[2] George Mason Univ, Manassas, VA 20110 USA

来源：

ENVIRONMENTAL MICROBIOLOGY | 2005年 / 397卷

基金：

美国国家科学基金会;

关键词：

D O I：

10.1016/S0076-6879(05)97006-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Methods are provided for the culture of neutrophilic Fe-oxidizing bacteria (FeOB) that grow under microaerobic and anaerobic conditions. For oxygen-requiring lithotrophic Fe oxidizers, it is essential that both O-2 and Fe(II) concentrations are kept low, but that an adequate flux of both O-2 and Fe(II) are provided to support growth. Techniques using opposing gradients of Fe(II) and O-2 are discussed. Preparation of stock solutions of FeS and FeCl2 are described. Methods for the culture of anaerobic FeOB that utilize nitrate or light (anoxygenic photosynthesis) are discussed and presented briefly.

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收藏

页码：112 / 123

页数：12

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