Isotopic fractionation of carbon and chlorine by microbial degradation of dichloromethane

被引：85

作者：

Heraty, LJ ^{[1
]}

Fuller, ME ^{[1
]}

Huang, L ^{[1
]}

Abrajano, T ^{[1
]}

Sturchio, NC ^{[1
]}

机构：

[1] Argonne Natl Lab, Div Environm Res, Argonne, IL 60439 USA

来源：

ORGANIC GEOCHEMISTRY | 1999年 / 30卷 / 8A期

关键词：

D O I：

10.1016/S0146-6380(99)00062-5

中图分类号：

P3 [地球物理学]; P59 [地球化学];

学科分类号：

0708 ; 070902 ;

摘要：

Microbial culture experiments were performed at 22 degrees C to investigate isotopic fractionation of carbon (C) and chlorine (Cl) during aerobic degradation of dichloromethane (DCM) by MC8b, a gram-negative methylotrophic organism closely related to the genera Methylobacterium or Ochrobactrum. Values of the isotopic fractionation factor, alpha, were estimated from the experimental results. These values are 0.9576 +/- 0.0015 for C and 0.9962 +/- 0.0003 for Cl, corresponding to kinetic isotope effects (KIE) of 1.0424 for C and 1.0038 for Cl. The C isotope effect accompanying aerobic degradation of DCM by MC8b is roughly twice that resulting from microbial oxidation of CH4. Further studies of this type, for a variety of chlorinated aliphatic hydrocarbons (CAHs) under a range of biogeochemical conditions, may find important applications in the characterization and remediation of sites contaminated with CAHs. (C) 1999 Elsevier Science Ltd. All rights reserved.

引用

页码：793 / 799

页数：7

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