Behavioral dysfunction, brain oxidative stress, and impaired mitochondrial electron transfer in aging mice

被引:196
作者
Navarro, A
Del Pino, MJS
Gómez, C
Peralta, JL
Boveris, A
机构
[1] Univ Cadiz, Fac Med, Dept Bioquim & Biol Mol, Cadiz 11003, Spain
[2] Univ Cadiz, Fac Med, Dept Biostat, Cadiz 11003, Spain
[3] Univ Buenos Aires, Sch Pharm & Biochem, Lab Free Rad Biol, RA-1113 Buenos Aires, DF, Argentina
关键词
neuromuscular impairment; NADH-cytochrome c reductase;
D O I
10.1152/ajpregu.00537.2001
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
Behavioral tests, tightrope success, and exploratory activity in a T maze were conducted with male and female mice for 65 wk. Four groups were defined: the lower performance slow males and slow females and the higher performance fast males and fast females. Fast females showed the longest life span and the highest performance, and slow males showed the lowest performance and the shortest life span. Oxidative stress and mitochondrial electron transfer activities were determined in brain of young (28 wk), adult (52 wk), and old (72 wk) mice in a cross-sectional study. Brain thiobarbituric acid reactive substances (TBARS) were increased by 50% in old mice and were similar to15% higher in males than in females and in slow than in fast mice. Brain Cu, Zn-superoxide dismutase (SOD) activity was increased by 52% and Mn-SOD by 108% in old mice. The activities of mitochondrial enzymes NADH-cytochrome c reductase, cytochrome oxidase, and citrate synthase were decreased by 14-58% in old animals. The cumulative toxic effects of oxyradicals are considered the molecular mechanism of the behavioral deficits observed on aging.
引用
收藏
页码:R985 / R992
页数:8
相关论文
共 39 条
[1]  
[Anonymous], HUMAN PHYSL AGE STRE
[2]   THE MITOCHONDRIAL ELECTRON-TRANSFER ALTERATION AS A FACTOR INVOLVED IN THE BRAIN AGING [J].
BENZI, G ;
PASTORIS, O ;
MARZATICO, F ;
VILLA, RF ;
DAGANI, F ;
CURTI, D .
NEUROBIOLOGY OF AGING, 1992, 13 (03) :361-368
[3]   MPP+-induced mitochondrial dysfunction is potentiated by dopamine [J].
Boada, J ;
Cutillas, B ;
Roig, T ;
Bermúdez, J ;
Ambrosio, S .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2000, 268 (03) :916-920
[4]   MEASUREMENT OF CYTOCHROMES IN HUMAN SKELETAL-MUSCLE MITOCHONDRIA, ISOLATED FROM FRESH AND FROZEN STORED MUSCLE SPECIMENS [J].
BOOKELMAN, H ;
TRIJBELS, JMF ;
SENGERS, RCA ;
JANSSEN, AJM .
BIOCHEMICAL MEDICINE, 1978, 19 (03) :366-373
[5]  
Boveris A, 2000, ANN NY ACAD SCI, V899, P121
[6]  
BOVERIS A, 1999, OXIDAT STRESS DIS, V2, P1
[7]   HYDROPEROXIDE METABOLISM IN MAMMALIAN ORGANS [J].
CHANCE, B ;
SIES, H ;
BOVERIS, A .
PHYSIOLOGICAL REVIEWS, 1979, 59 (03) :527-605
[8]  
Clark JB, 1997, NEUROCHEMISTRY PRACT, P151
[9]  
Cutler R., 1984, Free Radicals in Biology, V6, P371
[10]   Relation between exploratory activity and immune function in aged mice:: a preliminary study [J].
De la Fuente, M ;
Miñano, M ;
Victor, VM ;
Del Rio, M ;
Ferrández, MD ;
Díez, A ;
Miquel, J .
MECHANISMS OF AGEING AND DEVELOPMENT, 1998, 102 (2-3) :263-277