Study on chemical fingerprinting of crude and processed Atractylodes macrocephala from different locations in Zhejiang province by reversed-phase high-performance liquid chromatography coupled with hierarchical cluster analysis

被引：30

作者：

Cai, Hao ^{[1
,2
,4
]}

Xu, Zhiwei ^{[3
]}

Luo, Sucai ^{[3
]}

Zhang, Wenwen ^{[3
]}

Cao, Gang ^{[3
]}

Liu, Xiao ^{[1
,2
,4
]}

Lou, Yajing ^{[1
,2
]}

Ma, Xiaoqing ^{[1
,2
]}

Qin, Kunming ^{[1
,2
]}

Cai, Baochang ^{[1
,2
,3
,4
]}

机构：

[1] Nanjing Univ Chinese Med, Ctr Engn, State Minist Educ Standardizat Chinese Med Proc, Nanjing 210029, Jiangsu, Peoples R China

[2] Nanjing Univ Chinese Med, Coll Pharm, Dept Chinese Mat Med, Nanjing 210046, Jiangsu, Peoples R China

[3] Zhejiang Chinese Med Univ, Res Ctr TCM Proc Technol, Hangzhou 310053, Zhejiang, Peoples R China

[4] Nanjing Univ Chinese Med, Natl Class Key Discipline Sci Chinese Mat Med 1, Nanjing 210046, Jiangsu, Peoples R China

来源：

PHARMACOGNOSY MAGAZINE | 2012年 / 8卷 / 32期

基金：

中国国家自然科学基金;

关键词：

Atractylodes macrocephala; chemical fingerprinting; hierarchical cluster analysis; high-performance liquid chromatography; processed; CHINA; KOIDZ;

D O I：

10.4103/0973-1296.103659

中图分类号：

R914 [药物化学];

学科分类号：

100705 [微生物与生化药学];

摘要：

Background: In China, Atractylodes macrocephala is mainly distributed in Zhejiang province. It is, therefore, desirable to determine a reliable and accurate methodology to differentiate the samples collected from Zhejiang province. Although some studies on the fingerprints of Atractylodes macrocephala using HPLC have been published, none of them compared the processed product of Atractylodes macrocephala from different areas of Zhejiang province. Materials and Methods: Reversed-phase high-performance liquid chromatography (RP-HPLC) coupled with hierarchical cluster analysis was employed in the fingerprint analysis of Atractylodes macrocephala from Zhejiang province, China. The LC assay was performed on a reversed-phase C-18 column with linear gradient elution using water and acetonitrile. The LC data showed considerable variation of chemical constituents among Atractylodes macrocephala populations. Results: 21 and 22 characteristic peaks in the 14 grants of Atractylodes macrocephala and its processed product were determined in samples from different habitats of Zhejiang province, respectively. Their chromatographic patterns were generally consistent although their contents of chemical compositions were greatly different. The results of hierarchical cluster analysis showed that the samples could be divided into four groups; it was able to select excellent resources from the groups. Conclusion: This was the first report of hierarchical cluster analysis of crude and processed Atractylodes macrocephala according to their chemical fingerprints and could be applied to the intrinsic quality control of crude and processed Atractylodes macrocephala. The processing technique of Atractylodes macrocephala through the pilot-scale experiment was first studied and is simple and suitable for prepared yin plan of Atractylodes macrocephala industrial manufacture.

引用

页码：300 / 307

页数：8

共 18 条

[1]

THE ACETYLENES FROM ATRACTYLODES-MACROCEPHALA [J].