Lectin from Canavalia brasiliensis Seeds (ConBr) Is a Valuable Biotechnological Tool to Stimulate the Growth of Rhizobium tropici in Vitro

被引：11

作者：

de Vasconcelos, Mayron Alves ^{[1
]}

Cunha, Claudio Oliveira ^{[1
]}

Sousa Arruda, Francisco Vassiliepe ^{[2
]}

Carneiro, Victor Alves ^{[2
]}

Mercante, Fabio Martins ^{[4
]}

do Nascimento Neto, Luiz Gonzaga ^{[2
]}

de Sousa, Giselly Soares ^{[3
]}

Matias Rocha, Bruno Anderson ^{[1
]}

Teixeira, Edson Holanda ^{[2
]}

Cavada, Benildo Sousa ^{[1
]}

dos Santos, Ricardo Pires ^{[3
]}

机构：

[1] Univ Fed Ceara, Dept Biochem & Mol Biol, BR-60440970 Fortaleza, CE, Brazil

[2] Univ Fed Ceara, Sch Med, Integrated Lab Biomol LIBS, BR-62042280 Sobral, CE, Brazil

[3] Univ Fed Ceara, Comp Engn Biotechnol Ctr Sobral, BR-62011000 Fortaleza, Ceara, Brazil

[4] Embrapa Western Region Agr, BR-79804970 Dourados, MS, Brazil

来源：

MOLECULES | 2012年 / 17卷 / 05期

关键词：

lectin; Canavalia brasiliensis; Canavalia ensiformis; Rhizobium tropici; interaction; growth; BIOLOGICAL-ACTIVITY; CRYSTAL-STRUCTURE; LEGUME;

D O I：

10.3390/molecules17055244

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

To study the interactions between a Rhizobium tropici strain and lectins isolated from the seeds of Canavalia ensiformis (ConA) and Canavalia brasiliensis (ConBr), a lectin fluorescence assay was performed. In addition, an experiment was designed to evaluate the effect of the two lectins on bacterial growth. Both lectins were found to bind to R. tropici cells, but the interactions were inhibited by D-mannose. Interestingly, only ConBr stimulated bacterial growth in proportion to the concentrations used (15.6-500 mu g/mL), and the bacterial growth stimulation was inhibited by D-mannose as well. Structure/Function analyses by bioinformatics were carried out to evaluate the volume and carbohydrate recognition domain (CRD) configuration of ConA and ConBr. The difference of spatial arrangement and volume of CRD may indicate the variation between biological activities of both lectins. The results suggest that ConBr could be a promising tool for studies focusing on the interactions between rhizobia and host plants.

引用

页码：5244 / 5254

页数：11

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