Multiparameter evanescent-wave Imaging in biological fluorescence microscopy

被引：11

作者：

Oheim, M ^{[1
]}

Stühmer, W

机构：

[1] Ecole Super Phys & Chem, F-75005 Paris, France

[2] Max Planck Inst Expt Med, D-37073 Gottingen, Germany

来源：

IEEE JOURNAL OF QUANTUM ELECTRONICS | 2002年 / 38卷 / 02期

关键词：

fluorescence; fluorescence quenching; polarization; TIRF; variable angle;

D O I：

10.1109/3.980266

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

Wide-field optical microscopy of live cells with near-field precision, tracking the dynamics of subcellular organelles, imaging of single-molecule reactions with millisecond time-resolution, or watching synaptic nerve terminals "in action"-these are some examples of recent work that triggered the "renaissance" of evanescent-wave (EW) spectroscopy in biological imaging. In these studies, the goal is to markedly reduce background fluorescence from locations in the sample other than the cell/substrate interface. After a brief reminder of EW generation, we discuss how the confinement of fluorescence excitation highlights cellular structure near the plasma membrane with unprecedented detail. We then illustrate how the intensity distribution and polarization of the EW can be used to study dynamic biological processes that have neither been accessible with optical (confocal or two-photon fluorescence) nor electron microscopy, and take a glimpse of what is to come in EW imaging.

引用

页码：142 / 148

页数：7

共 71 条

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