The transmembrane domain directs TLR9 to intracellular compartments that contain TLR3

被引:32
作者
Kajita, E [1 ]
Nishiya, T [1 ]
Miwa, S [1 ]
机构
[1] Hokkaido Univ, Grad Sch Med, Dept Cellular Pharmacol, Sapporo, Hokkaido 0608638, Japan
关键词
intracellular localization; toll-like receptor 9; transmembrane domain;
D O I
10.1016/j.bbrc.2006.03.014
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Toll-like receptors (TLRs) are type I transmembrane (TM) proteins indispensable for sensing microbial and viral infection. Despite their conserved primary structures, some TLRs that detect pathogen-derived nucleic acids (TLR3, TLR7, TLR8, and TLR9) are retained in the cytoplasm. The intracellular localization of TLR9 is important for its ability to discriminate self- and non-scif DNA, but the mechanism by which it is retained in the cytoplasm is unclear. In the present study, we found that the TM domain of TLR9 directs its intracellular localization. The TM domain of TLR9 also targets CD25, a heterologous type I TM protein, to intracellular compartments that contain TLR9. We also found that TLR9 generally co-localizes with TLR3, although its linker region, not its TM domain, directs intracellular localization of TLR3. These data demonstrate that the TM domain of TLR9 is a critical regulatory element that targets TLR9 to its intracellular location. (c) 2006 Elsevier Inc. All rights reserved.
引用
收藏
页码:578 / 584
页数:7
相关论文
共 21 条
  • [1] Recognition of double-stranded RNA and activation of NF-κB by Toll-like receptor 3
    Alexopoulou, L
    Holt, AC
    Medzhitov, R
    Flavell, RA
    [J]. NATURE, 2001, 413 (6857) : 732 - 738
  • [2] Intracellular localization of Toll-like receptor 9 prevents recognition of self DNA but facilitates access to viral DNA
    Barton, GM
    Kagan, JC
    Medzhitov, R
    [J]. NATURE IMMUNOLOGY, 2006, 7 (01) : 49 - 56
  • [3] The cytoplasmic 'linker region' in Toll-like receptor 3 controls receptor localization and signaling
    Funami, K
    Matsumoto, M
    Oshiumi, H
    Akazawa, T
    Yamamoto, A
    Seya, T
    [J]. INTERNATIONAL IMMUNOLOGY, 2004, 16 (08) : 1143 - 1154
  • [4] Endoplasmic reticulum-mediated phagocytosis is a mechanism of entry into macrophages
    Gagnon, E
    Duclos, S
    Rondeau, C
    Chevet, E
    Cameron, PH
    Steele-Mortimer, O
    Paiement, J
    Bergeron, JJM
    Desjardins, M
    [J]. CELL, 2002, 110 (01) : 119 - 131
  • [5] Species-specific recognition of single-stranded RNA via toll-like receptor 7 and 8
    Heil, F
    Hemmi, H
    Hochrein, H
    Ampenberger, F
    Kirschning, C
    Akira, S
    Lipford, G
    Wagner, H
    Bauer, S
    [J]. SCIENCE, 2004, 303 (5663) : 1526 - 1529
  • [6] A Toll-like receptor recognizes bacterial DNA
    Hemmi, H
    Takeuchi, O
    Kawai, T
    Kaisho, T
    Sato, S
    Sanjo, H
    Matsumoto, M
    Hoshino, K
    Wagner, H
    Takeda, K
    Akira, S
    [J]. NATURE, 2000, 408 (6813) : 740 - 745
  • [7] TLR9 signals after translocating from the ER to CpG DNA in the lysosome
    Latz, E
    Schoenemeyer, A
    Visintin, A
    Fitzgerald, KA
    Monks, BG
    Knetter, CF
    Lien, E
    Nilsen, NJ
    Espevik, T
    Golenbock, DT
    [J]. NATURE IMMUNOLOGY, 2004, 5 (02) : 190 - 198
  • [8] Chromatin-IgG complexes activate B cells by dual engagement of IgM and Toll-like receptors
    Leadbetter, EA
    Rifkin, IR
    Hohlbaum, AM
    Beaudette, BC
    Shlomchik, MJ
    Marshak-Rothstein, A
    [J]. NATURE, 2002, 416 (6881) : 603 - 607
  • [9] TLR9 Is localized in the endoplasmic reticulum prior to stimulation
    Leifer, CA
    Kennedy, MN
    Mazzoni, A
    Lee, CW
    Kruhlak, MJ
    Segal, DA
    [J]. JOURNAL OF IMMUNOLOGY, 2004, 173 (02) : 1179 - 1183
  • [10] Toll-like receptor 9-mediated recognition of herpes simplex virus-2 by plasmacytoid dendritic cells
    Lund, J
    Sato, A
    Akira, S
    Medzhitov, R
    Iwasaki, A
    [J]. JOURNAL OF EXPERIMENTAL MEDICINE, 2003, 198 (03) : 513 - 520