Comparative study of electron mediators used in the electrochemical oxidation of NADH

被引：79

作者：

Prieto-Simón, B ^{[1
]}

Fàbregas, E ^{[1
]}

机构：

[1] Univ Autonoma Barcelona, Dept Quim, Grup Sensors & Biosensors, E-08193 Barcelona, Spain

来源：

BIOSENSORS & BIOELECTRONICS | 2004年 / 19卷 / 10期

关键词：

redox mediators; chemically modified electrodes; amperometric detection of NADH;

D O I：

10.1016/j.bios.2003.10.010

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

A comparison of the behavior of six different redox mediators for the electrocatalysis of the oxidation of NADH, which are widely used in the construction of dehydrogenase-based biosensors, is reported. The redox mediators were, potassium hexacyanoferrate (11), Meldola's Blue (MB), dichlorophenolindophenol (DCPIP), p-benzoquinone (p-BQ), o-phenylenediamine (o-PDA) and 3,4-dihydroxybenzaldehyde (3,4-DHB). After incorporating each of them in the sensor system following four different strategies (in solution, entrapped in epoxy-composites, adsorbed or electropolymerized on the electrode surface), several aspects regarding repeatability and reproducibility were considered and compared. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：1131 / 1138

页数：8

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