Gene transfer mediated by YKS-220 cationic particles: Convenient and efficient gene delivery reagent

被引：8

作者：

Yu, W ^{[1
]}

Shimoyama, A ^{[1
]}

Uneda, T ^{[1
]}

Obika, S ^{[1
]}

Miyashita, K ^{[1
]}

Doi, T ^{[1
]}

Imanishi, T ^{[1
]}

机构：

[1] Osaka Univ, Grad Sch Pharmaceut Sci, Osaka 5650871, Japan

来源：

JOURNAL OF BIOCHEMISTRY | 1999年 / 125卷 / 06期

关键词：

cationic liposomes; cationic particles; transfection;

D O I：

10.1093/oxfordjournals.jbchem.a022383

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A monocationic lipid, YKS-220, with a symmetrical and biodegradable structure can be used as an effective gene transfer vector in a cationic particle form (not a cationic liposome form), and is obtained by diluting an ethanol solution of YKS-220 and DOPE (1:5, molar ratio) with an aqueous medium. This preparation method is more convenient than that for cationic liposomes, YKS-220 cationic particles showed a heterogeneous large mean diameter of 4.4 mu m. An obvious size change was not observed when plasmid DNA was added. The transfection activity of YKS-220 cationic particles was comparable to those of YKS-220 liposomes and DOSPA liposomes (LipofectAMINE(TM)), and even higher than that of DOGS (TRNSFECTAM), Interestingly, the YKS-220 cationic particle/DNA complexes were resistant to the neutralizing effect of serum. All of these findings indicate that YKS-220 cationic particles are a convenient and efficient gene delivery reagent.

引用

页码：1034 / 1038

页数：5

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