Association of peroxisome proliferator activated receptor-γ gene with non-alcoholic fatty liver disease in Asian Indians residing in north India

被引:28
作者
Bhatt, Surya Prakash [1 ,2 ,6 ,7 ]
Nigam, Priyanka [4 ,5 ,7 ]
Misra, Anoop [4 ,5 ,7 ]
Guleria, Randeep [2 ]
Luthra, Kalpana [1 ]
Pandey, Ravindra Mohan [3 ]
Pasha, M. A. Qadar [6 ]
机构
[1] All India Inst Med Sci, Dept Biochem, New Delhi 110029, India
[2] All India Inst Med Sci, Dept Pulm Med & Sleep Disorders, New Delhi, India
[3] All India Inst Med Sci, Dept Biostat, New Delhi, India
[4] Natl Diabet Obes & Cholesterol Fdn NDOC, New Delhi, India
[5] Diabet Fdn India, New Delhi, India
[6] Inst Genom & Integrat Biol, Delhi, India
[7] Fortis C DOC Ctr Excellence Diabet Metab Dis & En, New Delhi, India
关键词
Non-alcoholic fatty liver disease; Peroxisome proliferator activated receptor-gamma; Haplotype; Asian Indians; INSULIN-RESISTANCE; METABOLIC SYNDROME; PRO12ALA POLYMORPHISM; OBESITY; SUSCEPTIBILITY;
D O I
10.1016/j.gene.2012.09.067
中图分类号
Q3 [遗传学];
学科分类号
071007 [遗传学];
摘要
Background: Genetics of non-alcoholic fatty liver (NAFLD) in Asian Indians has been inadequately studied. We investigated the association of polymorphisms C161T and Pro12Ala of peroxisome proliferator-activated receptor gamma (PPAR gamma) with clinical and biochemical parameters in Asian Indians with NAFLD. Methods: In this case-control study, 162 NAFLD cases and 173 controls were recruited. Abdominal ultrasound, clinical and biochemical profiles, fasting insulin levels and value of homeostasis model assessment of insulin resistance were determined. Polymerase chain reaction-restriction fragment length polymorphisms of two polymorphisms were performed. The association of these polymorphisms with clinical and biochemical parameters was analysed. Results: Higher frequency of Ala and T alleles of PPAR gamma was obtained in cases. Ala/Ala genotype of PPAR gamma (Pro12Ala) was associated with significantly higher serum triglycerides (TG), alkaline phosphatase (ALK) and waist-hip ratio in cases as compared to controls. In C161T polymorphism, IF genotype was significantly increased TG (p=0.04), total cholesterol (p=0.01), ALK (p=0.04) and gamma-glutamyl transpeptidase (p=0.007) in cases. The linkage disequilibrium for these two single-nucleotide polymorphisms of PPAR gamma was differed in cases (D1=0.1; p=0.006) and controls (D1=0.07; p=0.1). Using a multivariate analysis after adjusting for age, sex and body mass index, the presence of NAFLD was linked to these two polymorphisms (odds ratio 1.64 (95% CI: 1.09-2.45, p=0.05)]. Conclusion: Asian Indians in north India carrying the alleles Ala and T of PPAR gamma (Pro12Ala and C161T) polymorphisms are predisposed to develop NAFLD. (C) 2012 Elsevier B.V. All rights reserved.
引用
收藏
页码:143 / 147
页数:5
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