Preparation of highly purified momordin II without ribonuclease activity

被引：21

作者：

Valbonesi, P ^{[1
]}

Barbieri, L ^{[1
]}

Bolognesi, A ^{[1
]}

Bonora, E ^{[1
]}

Polito, L ^{[1
]}

Stirpe, F ^{[1
]}

机构：

[1] Univ Bologna, Dipartimento Patol Sperimentale, I-40126 Bologna, Italy

来源：

LIFE SCIENCES | 1999年 / 65卷 / 14期

关键词：

momordin; ribosome-inactivating proteins; polynucleotide : adenosine glycosidase; ribonuclease;

D O I：

10.1016/S0024-3205(99)00389-6

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Momordin II, a ribosome-inactivating protein from Momordica charantia seeds, was purified by a procedure involving a series of chromatographies on S-Sepharose, Sephadex G-50, CM-Sepharose, and Red Sepharose columns. Highly purified momordin II inhibited cell-free protein synthesis, released adenine from rat liver ribosomes and from DNA, and had no RNase activity.

引用

页码：1485 / 1491

页数：7

共 21 条

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